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目的:探讨血管紧张素转化酶(ACE)基因I/D多态性和载脂蛋白B(apoB)基因XbaI多态性对子痫前期子痫及其左心室损害的影响。方法:用聚合酶链反应-限制性内切酶片段长度多态性(PCR -RFLP)技术,检测103例子痫前期子痫患者及76例正常晚期妊娠妇女(对照组)的ACE基因I/D多态性和apoB基因XbaI多态性位点频率(两组孕妇的年龄及孕周相匹配),并对子痫前期子痫患者进行二维超声引导的M型超声检查。结果:子痫前期子痫组与对照组基因型与等位基因频率比较差异均无显著性(P>0 05)。但X+X- -(DD.ID)基因型与子痫前期子痫有关联(P<0 .05)。经校正其他因素影响后,子痫前期子痫组DD基因型患者左室重量指数和室壁相对厚度均高于II基因型患者(P<0 .05),经多元线性逐步回归分析,ACE基因型与左室重量指数和室壁相对厚度均独立相关,分别可解释左室重量指数和室壁相对厚度总变异的1 .76%和1 .4%。结论:X+X- -(DD+ID)基因型可能促进子痫前期子痫发生,并且ACE基因I/D多态性与其左室损害程度独立相关。
Objective: To investigate the effects of angiotensin I converting enzyme (ACE) gene I / D polymorphism and apolipoprotein B (XbaI) polymorphism on preeclampsia and left ventricular injury. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the ACE gene I / D in 103 preeclampsia patients and 76 normal pregnant women (control group) Polymorphism and XbaI polymorphism of apoB gene locus (two groups of pregnant women age and gestational age match), and preeclampsia eclampsia patients with two-dimensional ultrasound-guided M-mode ultrasound. Results: There was no significant difference in genotype and allele frequency between preeclampsia and control group (P> 0.05). However, the genotype X + X- (DD.ID) was associated with preeclampsia (P <0.05). After adjusting for other factors, the left ventricular mass index and the relative wall thickness of DD genotype in preeclampsia group were higher than those in genotype II genotype (P <0.05). After multivariate linear stepwise regression analysis, ACE genotype And left ventricular mass index and relative wall thickness were independently correlated, respectively, to explain the left ventricular mass index and the relative wall thickness of the total variation of 1.76% and 1.4%. Conclusion: The genotype X + X- (DD + ID) may promote the development of preeclampsia, and the ACE gene I / D polymorphism is independent of the degree of left ventricular injury.