用蛋白工程的方法，合成了庚型肝炎病毒（ＧＢＶＣ／ＨＧＶ）ＮＳ３区一段多肽，并用该条多肽初步建立了抗ＧＢＶＣ／ＨＧＶ酶联免疫试验方法（ＥＩＡ）。用该法检测我国部分地区２８７０份ＨＢｓＡｇ和抗ＨＣＶ均阴性的健康献血员血清。结果：抗ＧＢＶＣ／ＨＧＶ的流行率为３．４８％（１００／２８７０）。其中１００份抗ＧＢＶＣ／ＨＧＶ阳性血清用逆转录套式聚合酶链反应法（ＲＴＰＣＲ）检测，ＧＢＶＣ／ＨＧＶＲＮＡ的阳性率为６２．０％（６２／１００）。 A polypeptide of NS3 region of hepatitis C virus (GBVC / HGV) was synthesized by protein engineering method and the anti-GBVC / HGV ELISA method was initially established using this peptide. This method was used to test 2870 HBsAg and anti-HCV negative serum of healthy donors in some areas of our country. Results: The prevalence of anti-GBV C / HGV was 3.48% (100/2870). Among them, 100 positive anti-GBVC / HGV sera were detected by RTPCR, and the positive rate of GBVC / HGVRNA was 62.0% (62/100).