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目的:探讨不同来源冻融精子行卵胞浆内单精子注射(ICSI)的效果。方法:回顾性分析因无精症采用睾丸和附睾精子行ICSI的895个周期,其中诊断性附睾活检冻融精子7个周期(A组),诊断性睾丸活检组织冻融11个周期(B组),附睾穿刺取精(PESA)行ICSI后剩余精子冻融13个周期(C组),睾丸穿刺取精(TESA)行ICSI后剩余精子冻融12个周期(D组),PESA新鲜精子630个周期(E组),TESA新鲜精子222个周期(F组)。结果:6组的平均MⅡ卵子数、平均优质胚胎数比较,差异均无统计学意义(F=0.326、1.513,P>0.05),优质胚胎率、种植率、临床妊娠率差异亦没有统计学意义(χ2=4.874、2.766、1.617,P>0.05)。结论:不同来源冻融精子行ICSI可以获得与新鲜精子相似的临床结局。
Objective: To investigate the effect of intracytoplasmic sperm injection (ICSI) on frozen-thawed sperm from different sources. Methods: A retrospective analysis of azoospermia testis and epididymal sperm ICIC 895 cycles, including frozen-thawed diagnosis of epididymal sperm seven cycles (A group), diagnostic testis tissue freeze-thaw cycles of 11 cycles (B group ), Residual sperm freezing and thawing for 13 cycles (group C) after ICSI in epididymal puncture and sperm injection (PESA) group, remaining sperm freezing and thawing for 12 cycles (group D) after ICSI in testis puncture and spermatogenesis, PESA fresh sperm 630 A cycle (E group), TESA fresh sperm 222 cycles (F group). Results: There was no significant difference in the average number of M Ⅱ eggs and the average number of high quality embryos in the 6 groups (F = 0.326, 1.513, P> 0.05). There was also no significant difference in the rate of high quality embryos, implantation rate and clinical pregnancy rate (χ2 = 4.874,2.766,1.617, P> 0.05). CONCLUSIONS: ICSI from different sources of frozen-thawed sperm can achieve similar clinical outcomes as fresh sperm.