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[目的]研究α-氯代醇对大鼠的主要亚慢性毒性作用,确定毒作用靶器官及其毒作用剂量。[方法]参照GB15670-1995《农药登记毒理学试验方法》中大鼠亚慢性毒性经口毒性试验方法进行,设0,15,30,75mg/kg4个剂量组。[结果]与对照组比较,雄性高剂量组(75mg/kg)体重增长明显减慢(P﹤0.01),两性别高剂量组血常规中的血红蛋白含量和RBC计数均较对照组降低(P﹤0.05),两性别中、高剂量组肾脏脏器系数有明显改变(P﹤0.05或P﹤0.01),雄性中、高剂量组睾丸及两性别中、高剂量肾脏存在明显病理改变,雄性各剂量组精子计数、精子活动度明显降低(P﹤0.05或P﹤0.01)。[结论]在本试验条件下,连续摄入30mg/kg以上≥80%α-氯代醇90d对SD大鼠肾脏有显著损伤,连续摄入15mg/kg≥80%α-氯代醇90d对雄性SD大鼠精子活动度及精子计数就有显著影响,连续摄入30mg/kg以上≥80%α-氯代醇90d对雄性SD大鼠生殖系统有显著病理损伤。
[Objective] To study the main sub-chronic toxic effects of α-chlorohydrin on rats and to determine the toxic target organ and its toxic dose. [Method] With reference to the sub-chronic toxicity oral toxicity test method of GB15670-1995 “Test Method for Registration of Pesticide Registration Toxicity”, four dose groups of 0, 15, 30 and 75 mg / kg were set up. [Results] Compared with the control group, the body weight of the male high dose group (75 mg / kg) slowed down significantly (P <0.01). The hemoglobin content and RBC count of the high dose group and the high dose group were lower than those of the control group (P < 0.05). There was a significant change in the index of kidney organ between middle and high dose groups (P <0.05 or P <0.01). There were obvious pathological changes in medium and high dose kidney of testis, male and female, Group sperm count, sperm motility was significantly reduced (P <0.05 or P <0.01). [Conclusion] Under the test conditions, continuous ingestion of ≥80% α-chlorohydrin for 90 days caused significant damage to the kidneys of SD rats. Continuous ingestion of 15 mg / kg ≥80% α- Sperm motility and sperm count in male SD rats had a significant effect. Continuous intake of ≥80% α-chlorohydrin for 90 days had significant pathological damage to the reproductive system of male SD rats.