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为了明确镰刀菌属(Fusarium)美丽组(Section Elegans)中尖孢镰刀菌(F.oxysporum)和芬芳镰刀菌(F.redolens)2种菌的遗传差异性和亲缘关系,利用ISSR分子标记技术对这2种菌的35个菌株进行了遗传多样性分析。结果表明,用筛选的15条引物对35个供试菌株共扩增出231条条带,其中多态性条带220条,平均多态性比率为95.2%,平均每条引物产生条带为14.7条。聚类结果和遗传相似系数分析显示,35个菌株间的遗传相似系数范围为0.506~0.935,平均为0.661。在遗传相似系数为0.593时,供试的35株镰刀菌可明显的分成2个ISSR类群(IG),其中IGⅠ包括1~23号菌株,全部为F.oxysporum;IGⅡ包括24~35号菌株,全部为F.redolens。ISSR类群划分与菌种分类之间存在一定相关性(IGⅠ中23株F.oxysporum间的平均相似系数为0.720,IGⅡ中12株F.redolens间的平均相似系数为0.717),但与菌株的地理来源不存在相关性。而同一类群中,菌株之间的遗传相似性与菌株的地理来源存在一定的相关性。
In order to clarify the genetic diversity and genetic relationship of two species of F. oxysporum and F. redolens in the Fusarium beautiful Section (Section Elegans), ISSR molecular markers 35 strains of these 2 kinds of bacteria were analyzed for genetic diversity. The results showed that a total of 231 bands were amplified from 35 tested strains with 15 primers selected. Among them, 220 bands were polymorphic with the average ratio of 95.2%. The average band of each primer was Article 14.7. Cluster analysis and genetic similarity analysis showed that the genetic similarity coefficients of 35 isolates ranged from 0.506 to 0.935 with an average of 0.661. The 35 Fusarium species tested were significantly divided into two ISSR groups (IG) at the genetic similarity coefficient of 0.593, of which IGⅠ consisted of strains 1-23, all of which were F. oxysporum; IGⅡ included strains 24-35, All are F. redolens. There was a certain correlation between taxonomic classification and ISSR taxonomic classification (mean similarity coefficient of 0.720 between F. oxysporum in IGⅠ and 0.717 in F. redolens in IGⅡ) There is no correlation between sources. In the same group, the genetic similarity between strains is related to the geographical origin of the strains.