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目的 观察胎鼠宫内缺血缺氧后 ,线粒体与内质网钙离子 腺苷三磷酸酶 (Ca2 + ATPase)与钠离子 钾离子腺苷三磷酸酶 (Na+ K+ ATPase)的变化 ,及细胞内Ca2 + 超载与紊乱的机理。方法 制备胎鼠宫内不同程度缺血缺氧及再灌注模型 (缺血缺氧组 :缺血缺氧时间分别为 15、30、4 5、6 0min ;再灌注组 :缺血缺氧 15min后 ,分别再灌注 1、4、8、15、2 4h) ,各时间点分别有胎鼠 7～ 11只 ,假手术组胎鼠 12只。分离出胎鼠脑组织中线粒体与内质网 (匀浆后称微粒体 )行酶活性测定。结果 缺血缺氧组 :缺血缺氧 15、30、4 5、6 0min时 ,线粒体Ca2 + ATPase活性明显减弱 ,分别为 (6 1± 0 5 )、(5 7±0 8)、(5 7± 0 5 )、(5 4 1± 0 7) μmolPi/mg(蛋白·小时 ) ,差异有极显著性 (P <0 0 1) ;Na+ K+ ATPase活性也进行性降低 (P <0 0 1)。内质网Ca2 + ATPase活性变化不明显 (P >0 0 5 ) ,而Na+ K+ ATPase活性仍进行性降低 (P <0 0 1)。再灌注组 :1、4、8、15、2 4h时 ,线粒体Ca2 + ATPase活性明显减弱 ,分别为(7 0± 1 0 )、(6 8± 1 2 )、(7 5± 0 8)、(7 2± 0 9)、(6 7± 0 9) μmolPi/mg(蛋白·小时 ) ,差异有极显著性(P <0 0 1) ;内质网Ca2 + ATPase活性变化不明显 (P >0 0 5 ) ;Na+
Objective To observe the changes of Ca2 + ATPase and Na + K + ATPase in mitochondria and endoplasmic reticulum after intrauterine ischemia and hypoxia, Ca2 + overload and disorder mechanism. Methods The model of hypoxia-ischemia and reperfusion injury was established in fetal rats with different degrees of ischemia (hypoxia-ischemia group, ischemia-hypoxia time were 15, 30, 4, and 60 min, respectively. In reperfusion group, , Respectively, after reperfusion 1, 4, 8, 15, 24 h). There were 7-11 fetuses and 12 fetuses in sham operation group at each time point. Enzymatic activity of mitochondria and endoplasmic reticulum (microsomes after homogenization) were isolated from fetal brain tissue. Results In hypoxia-ischemia group, the activities of mitochondrial Ca2 + ATPase were significantly weakened at 15, 30, 45, 60 min after ischemia and hypoxia, respectively (6 1 ± 0 5, 5 7 ± 0 8, 5 (P <0.01). The activity of Na + K + ATPase also decreased progressively (P <0.01, P <0.01) ). Endoplasmic reticulum Ca2 + ATPase activity did not change significantly (P> 0.05), while Na + K + ATPase activity was still decreased (P <0.01). At 1, 4, 8, 15 and 24 h after reperfusion, the activities of mitochondrial Ca2 + ATPase in the reperfusion group were significantly decreased (70 ± 1 0, 66 ± 1 2, 75 ± 0 8, (P <0.01). The Ca2 + ATPase activity in endoplasmic reticulum was not significantly changed (P> 0.05) 0 0 5); Na +