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通过将免疫小鼠的脾细胞与HGPRT缺陷的NS－1骨髓瘤细胞进行细胞融合，并经HAT培养液对融合的杂交瘤细胞进行选择培养，再经二次限界稀释法对此杂交瘤细胞进行克隆化。采用无血清细胞培养技术，得到较高浓度而且较纯化的抗家兔晶体上皮细胞膜单克隆抗体。为进一步研究晶体上皮细胞的特性，以及利用免疫学方法抑制白内障术后晶体上皮细胞增殖奠定了基础。
The immunized mouse spleen cells were subjected to cell fusion with HGPRT-deficient NS-1 myeloma cells, and the hybridized hybridomas were subjected to selective culture in a HAT medium, followed by secondary limiting dilution to the hybridoma cells Clone. Serum-free cell culture technique was used to obtain higher concentration and more purified monoclonal antibody against rabbit lens epithelial cell membrane. This study laid the foundation for the further study of the characteristics of lens epithelial cells and the use of immunological methods to inhibit the proliferation of lens epithelial cells after cataract surgery.