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目的观察肿瘤坏死因子α(TNFα)对正常昆明小鼠骨骼肌和肝脏葡萄糖摄取的影响。方法80只昆明小鼠随机分为高剂量连续注射组(TH组,n=20)、TNFα低剂量连续注射组(TL组,n=20)、TNFα高剂量一次注射组(T1组,n=20)和正常对照组(C组,n=20),以3H标记的2脱氧葡萄糖(2-DG)为示踪剂,观察各组非胰岛素刺激(基础)和胰岛素刺激的离体骨骼肌和肝脏葡萄糖摄取量的变化。结果1)正常昆明小鼠肝脏基础葡萄糖摄取量明显高于骨骼肌葡萄糖摄取量(P<0.01),肝脏胰岛素刺激的葡萄糖摄取量变化值明显低于骨骼肌葡萄糖摄取量变化值(P<0.01)。2)TH组和TL组骨骼肌和肝脏基础葡萄糖摄取量均明显高于C组(P<0.01),且TH组明显高于TL组(P<0.01)。3)TH组和TL组骨骼肌和肝脏胰岛素刺激的葡萄糖摄取量均明显低于C组(P<0.01),且TH组明显低于TL组(P<0.01)。4)T1组骨骼肌和肝脏无论基础还是胰岛素刺激的葡萄糖摄取量均与C组差异无显著性(P>0.05)。结论1)正常动物肝脏和骨骼肌葡萄糖摄取方式不同,骨骼肌葡萄糖摄取受胰岛素调控比肝脏强。2)TNFα抑制组织胰岛素刺激的葡萄糖摄取,但促进组织基础葡萄糖摄取。3)TNFα对组织葡萄糖摄取的影响,呈剂量和时间依赖性。
Objective To observe the effect of tumor necrosis factor α (TNFα) on glucose uptake in skeletal muscle and liver of normal Kunming mice. Methods Totally 80 Kunming mice were randomly divided into high dose continuous injection group (TH group, n = 20), TNFα low dose continuous injection group (TL group, n = 20) 20 and normal control group (group C, n = 20). 3H-labeled 2-deoxyglucose (2-DG) was used as tracer to observe the effects of insulin-stimulated Changes in hepatic glucose uptake. Results 1) The liver basic glucose uptake of Kunming mice was significantly higher than that of skeletal muscle (P <0.01), and the change of hepatic insulin stimulated glucose uptake was significantly lower than that of skeletal muscle glucose uptake (P <0.01) . 2) The basal glucose uptake in skeletal muscle and liver of TH group and TL group were significantly higher than that of C group (P <0.01), and TH group was significantly higher than TL group (P <0.01). 3) Insulin-stimulated glucose uptake in skeletal muscle and liver of TH group and TL group were significantly lower than that of C group (P <0.01), and TH group was significantly lower than TL group (P <0.01). 4) There was no significant difference between the T1 group and the C group in skeletal muscle and liver glucose uptake regardless of basal or insulin stimulated (P> 0.05). Conclusion 1) Normal animal liver and skeletal muscle glucose intake in different ways, skeletal muscle glucose uptake regulation by insulin stronger than the liver. 2) TNFα inhibits insulin-stimulated glucose uptake in tissues but promotes basal glucose uptake in tissues. 3) Effect of TNFα on glucose uptake in tissues in a dose and time dependent manner.