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以分子量100D和500D规格的透析袋做工具,对吴茱萸和吴茱萸汤水提醇沉部分进行透析纯化,分别得于<100D,100～500D以及>500D共3部分。利用高效液相色谱对不同时间透析处理过的部分化学成分进行分析,确定透析效率最佳时间点,并以此对血管活性进行体外实验。通过比较吴茱萸碱、异鼠李素和柠檬苦素3种成分在不同透析时间处理下100～500D区间内的含量,初步确定48h透析处理为最佳透析效率时间点。通过检测该时间吴茱萸汤处理组中6-姜酚、人参皂苷Re、人参皂苷Rg1和人参皂苷Rb1的含量,进一步验证48h为最佳处理时间。吴茱萸汤和吴茱萸>100D透析部分具有明显的缩血管活性,<100D透析部分对血管收缩无明显影响。表明利用不同孔径和透析袋对提取物进行样品前处理,并用于体外实验具有一定的可行性。
Using dialysis bags with molecular weight of 100D and 500D as tools, dialyzing purification was performed on the part of water extracted from Evodia rutaecarpa and Evodia rutaecu brutistemol, respectively, in three fractions of <100D, 100 ~ 500D and> 500D. Using high performance liquid chromatography (HPLC), the chemical components of dialysis treated at different times were analyzed to determine the optimal time point of dialysis efficiency, and then the in vitro experiments on vasoactivity were carried out. By comparing evodiamine, isorhamnetin and limonin three components in different dialysis treatment time range of 100 ~ 500D, initially determined 48h dialysis treatment for the best time point of dialysis efficiency. By testing the contents of 6-gingerol, ginsenoside Re, ginsenoside Rg1 and ginsenoside Rb1 in the Evodia Decoction group at this time, the best treatment time was 48h. Evodia Decoction and Evodia> 100D dialysis part has obvious vasoconstriction activity, <100D dialysis part has no significant effect on vasoconstriction. It is indicated that it is feasible to sample extract with different pore size and dialysis bag and to be used in vitro.