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采用分光光度法对喀什地区核桃叶中黄酮类化合物的总含量进行了测定.用70%乙醇多次超声提取黄酮类化合物,以芦丁为对照品,Na NO2-Al(NO3)3-Na OH为显色系统,在510 nm处进行分光光度测定,建立标准曲线回归方程A=12.432C+0.019,线性相关系数R2=0.9974,测定结果表明,核桃叶中黄酮类化合物的总含量为0.0346 mg/g.
The total content of flavonoids in walnut leaves of Kashgar region was determined by spectrophotometry. Flavonoids were extracted by multiple ultrasonic waves with 70% ethanol and rutin was used as reference substance. NaNO2-Al (NO3) 3-Na OH For the color system, spectrophotometry was carried out at 510 nm. The standard curve regression equation A = 12.432C + 0.019 and the linear correlation coefficient R2 = 0.9974 were established. The results showed that the total flavonoids content in walnut leaves was 0.0346 mg / g.