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目的 观察褐藻硫酸多糖对老年人肠道中双歧杆菌促增殖作用的影响。方法 选择青岛地区健康老年人 5 6例 ,随机分为两组 ,试验组和对照组各 2 8例 ,试验组口服褐藻硫酸多糖每人每天 2 .5 g(冷提取物 SPC和热提取物 SPH各 1.2 5 g) ,共口服 5 d,对照组不服用。试验组于口服前及口服后第 1、2、3、5、8、14天无菌操作留取粪便 ,定量进行 10倍系列稀释 ,采用滴注法接种于 BS和 EMB平板培养基上 ,分别于 35℃厌氧和需氧培养 48h,取出后分别计数双歧杆菌和大肠杆菌菌落数 ,对照组同步进行。结果在试验剂量下 ,褐藻硫酸多糖 (SP)能有效地扶持、促进健康老年人肠道内双歧杆菌的生长繁殖 ,试验组口服 SP后第 3天 ,粪便中的双歧杆菌数明显增多 ,竞争地抑制大肠杆菌 ,第 5天双歧杆菌增至 11.88± 0 .98,大肠杆菌降至 6 .6 1± 1.0 1,B/E值增加到 1.6 7± 0 .88,与口服前比 ,有显著性和非常显著性差异 (P<0 .0 5 ,P<0 .0 5 ,P<0 .0 1) ;结果还显示 ,试验组口服 SP后第 3、5天粪便中培养计数的双歧杆菌、大肠杆菌数及 B/E值与口服后第 8、14天 (即停服 SP的第 3、9天 )比 ,均无显著性差异 (P>0 .0 5 )。结论 褐藻硫酸多糖是一种有效的双歧因子。在试验剂量下 ,对受试者肠道中双歧杆菌有显著的增值作用 ,且有较好的?
Objective To observe the effects of fucoidan on the proliferation of bifidobacteria in the intestine of the elderly. Methods Fifty-six healthy elderly in Qingdao were selected and randomly divided into two groups. The experimental group and the control group had 28 cases each. The experimental group orally took 2 .5 g of brown alginate polysaccharide per day per person (SPC of cold extract and SPH Each 1.2 5 g), a total of oral 5 d, the control group did not take. The experimental group before oral administration and after oral administration on the 1st, 2nd, 3rd, 5th, 8th and 14th days aseptic operation to take stool, quantitative 10-fold serial dilutions were instilled in the BS and EMB plate medium, respectively Anaerobic and aerobic culture was carried out at 35 ℃ for 48h. After removal, the number of colonies of Bifidobacterium and Escherichia coli were counted, and the control group was carried out simultaneously. Results At the test dose, the brown algae sulfate polysaccharide (SP) could effectively support and promote the growth and reproduction of bifidobacteria in the healthy elderly. On the third day after oral administration of SP, the number of bifidobacteria in the test group increased obviously, and the competition , The amount of Bifidobacterium increased to 11.88 ± 0.98 on the fifth day, the value of Escherichia coli decreased to 6.61 ± 1.01, the value of B / E increased to 1.6 7 ± 0.88, (P <0.05, P <0.05, P <0.01). The results also showed that in the test group, the double There was no significant difference (P> 0.05) between the number of Bifidobacterium, Escherichia coli and B / E values on the 8th and 14th day after oral administration (ie, the 3rd and 9th day after stopping taking SP). Conclusion Brown algae sulfate polysaccharide is an effective bifidus factor. In the test dose, the subjects of intestinal bifidobacteria have a significant value-added role, and better?