论文部分内容阅读
鸟氨酸转氨酶是以鸟氨酸为前体合成脯氨酸的关键酶,对植物适应逆境胁迫起关键作用。本研究根据其已知保守序列设计兼并引物,从普通烟草品种中烟14中克隆到一条425bp的中间片段,应用RACE方法设计特异性引物扩增得到5′末端和3′末端cDNA序列,经BLAST验证,首次从普通烟草中克隆到了鸟氨酸转氨酶基因,命名为Ntδ-OAT,其GenBank登录号为GU144571。该基因cDNA全长为1781bp,共编码477个氨基酸。系统进化树分析显示,Ntδ-OAT基因的进化符合传统的生物学分类,主要功能域在生物进化中保守性较高。Real-time PCR分析表明,Ntδ-OAT基因受干旱、高盐、低温、ABA等多种胁迫处理的诱导表达,可能参与普通烟草体内抗渗透胁迫过程。
Ornithine transaminase is a key enzyme in the synthesis of proline with ornithine as a precursor, and plays a key role in plant adaptation to stress. In this study, a 425 bp fragment was cloned from Nicotiana tabacum (Nicotiana tabacum L.) according to its known conserved sequences. A 425 bp DNA fragment was cloned from Nicotiana tabacum (Nicotiana tabacum L.). The specific primers were amplified by RACE to obtain the 5 ’end and 3’ Validated for the first time, the ornithine aminotransferase gene was cloned from Nicotiana tabacum and named as Ntδ-OAT with GenBank accession number GU144571. The full-length cDNA of this gene is 1781bp, encoding a total of 477 amino acids. Phylogenetic tree analysis showed that the evolution of Ntδ-OAT gene conformed to the traditional biological classification, and the main functional domains were highly conserved in biological evolution. Real-time PCR analysis showed that Ntδ-OAT gene induced by drought, high salt, low temperature, ABA and other stress treatment may be involved in the anti-osmotic stress of common tobacco.