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应用原位分子杂交及免疫组化方法，使用地高辛标记ＨＣＶ５＇非编码区探针及抗ＨＣＶＮＳ３区Ｃ３３ｃ单克隆抗体检测３５例人原发性肝内胆管细胞癌，癌旁肝组织内ＨＣＶＲＮＡ及其ＮＳ３抗原，发现ＨＣＶＲＮＡ在肝内胆管细胞癌中的阳性率为８３％。ＨＣＶＲＮＡ定位于肝细胞胞浆中，个别病例在淋巴细胞、肝窦内皮细胞和枯否细胞中发现阳性信号。ＨＣＶＮＳ３区Ｃ３３ｃ抗原在肝内胆管细胞癌中的阳性率为８９％，阳性反应主要位于癌细胞胞浆内或肝细胞胞浆内，阳性细胞在癌组织中以灶性分布为主，在癌旁肝组织以弥漫分布为主。ＨＢ－ｘＡｇ在本组病例的阳性率为７７％。本文结果提示，在肝内胆管细胞癌的发生中，除ＨＢＶ以外，ＨＣＶ感染与其发生也有密切关系，是其致病因素之一。
Using in situ hybridization and immunohistochemistry, 35 cases of human primary intrahepatic cholangiocarcinoma were detected with a digoxigenin-labeled HCV 5’ non-coding region probe and anti-HCV NS3 region C33c monoclonal antibody. With its NS3 antigen, the positive rate of HCV RNA in intrahepatic cholangiocarcinoma was found to be 83%. HCV RNA is localized in the cytoplasm of hepatocytes, and positive signals were found in lymphocytes, sinusoidal endothelial cells, and Kupffer cells in individual cases. The positive rate of HCV NS3 C33c antigen in intrahepatic cholangiocarcinoma was 89%. The positive reaction was mainly located in the cytoplasm of the cancer cells or in the cytoplasm of the hepatocytes. The positive cells were focally distributed in the cancer tissues and adjacent to the cancer. The liver tissue is diffusely distributed. The positive rate of HB-xAg in this group was 77%. The results of this article suggest that in the occurrence of intrahepatic cholangiocarcinoma, in addition to HBV, HCV infection is also closely related to its occurrence and is one of its pathogenic factors.