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目的 探讨参麦注射液和氨茶碱对慢性缺氧大鼠膈肌细胞凋亡及Fas/FasL基因蛋白系统表达的影响。方法 雄性Wistar大鼠 75只 ,随机分为空白组 (A组 )、参麦组 (B组 )和氨茶碱组 (C组 ) ,每组 2 5只 ,根据实验要求 ,3组再各分为缺氧前、缺氧 1周、2周、3周及 4周组 ,每组 5只。各组缺氧浓度均为 ( 10± 3 ) % ,7d/w ,8h/d ;B、C组在缺氧前分别注射参麦 ( 2ml/只 )、氨茶碱 ( 10mg/kg)。采用免疫组织化学及末端脱氧核苷酸转移酶介导的三磷酸脱氧尿嘧啶缺口末端标记 (TUNEL)法 ,检测各实验组Fas/FasL系统的表达及膈肌细胞凋亡 ,比较参麦和氨茶碱干预后对上述指标的影响。结果 ( 1)A组膈肌细胞缺氧前Fas、FasL的表达阳性率分别为 ( 2 77± 0 45) %、( 2 3 2± 0 61) % ,缺氧后其表达随缺氧时间的延长而增加 ;经参麦干预后 ,B组在缺氧 1周、2周、3周、4周的Fas表达 [( 6 3 6±4 17) %、( 9 77± 4 12 ) %、( 18 0 2± 6 91) %、( 2 1 0 9± 8 0 9) % ]、FasL表达 [( 5 3 2± 6 16) %、( 9 58±3 79) %、( 12 0 1± 8 71) %、( 19 43± 10 3 1) % ]与A组同期比较差异有显著性 (P均 <0 0 5)。而氨茶碱对其表达的作用不明显 ,C组Fas表达 [( 10 87± 3 62 ) %、( 2 4 13± 3 79) %、( 3 5 3 9± 9 0
Objective To investigate the effects of shenmai injection and aminophylline on the apoptosis of diaphragm muscle cells and the expression of Fas / FasL gene protein in chronic hypoxic rats. Methods 75 male Wistar rats were randomly divided into blank group (group A), Shenmai group (group B) and aminophylline group (group C), 25 rats in each group. According to the experimental requirements, Before hypoxia, hypoxia 1 week, 2 weeks, 3 weeks and 4 weeks group, each group of 5. The hypoxia concentrations in each group were (10 ± 3)%, 7d / w and 8h / d respectively. Before the hypoxia, the rats in groups B and C were injected Shenmai (2ml / only) and aminophylline (10mg / kg) respectively. Immunohistochemistry and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) were used to detect the expression of Fas / FasL and the apoptosis of diaphragm cells in each experimental group. Alkali intervention after the impact of these indicators. Results (1) The positive expressions of Fas and FasL in diaphragm cells of group A were (2 77 ± 0 45)% and (2 3 2 ± 0 61)% respectively after hypoxia, (6 36 ± 4 17)%, (9 77 ± 4 12)%, (18 ± 18)% in group B after hypoxia for 1 week, 2 weeks, 3 weeks and 4 weeks There was no significant difference in the expression of FasL between the two groups (0 2 ± 6 91%, (2 1 0 9 ± 8 0 9)%], (5 32 ± 6 16%, (9 58 ± 3 79)%, (12 0 1 ± 8 71 ), (19 43 ± 10 3 1)%] compared with the same period in group A significant difference (all P <0 05). However, the effect of aminophylline on its expression was insignificant. The expression of Fas in group C [(10 87 ± 32 62)%, (24 41 ± 3 79)%, (35 59 ± 90