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目的研究羟基积雪草苷在大鼠肠段各段的吸收特征,探究其在肠道的吸收机制,为设计药物的剂型和给药途径提供理论基础。方法利用大鼠在体循环肠灌流模型和方法,探明羟基积雪草苷的肠道吸收部位和吸收特征,以及不同表面活性剂对其肠吸收的影响。结果羟基积雪草苷在大鼠小肠段吸收强于大肠段,其吸收速率回肠>空肠≥十二指肠>结肠。而不同浓度的羟基积雪草苷溶液回肠吸收率和吸收速率常数无显著差异。在3种不同表面活性剂中,聚山梨酯-80对羟基积雪草苷的回肠吸收具有显著的促进作用。结论羟基积雪草苷的肠吸收主要集中在小肠中段,且吸收机制推测为被动扩散,表面活性剂聚山梨酯-80可以促进其回肠吸收。
OBJECTIVE To study the absorption characteristics of Madecassoside in various segments of the intestine in rats and to explore its mechanism of absorption in the intestine and to provide a theoretical basis for the design of drug formulations and administration routes. Methods The systemic intestinal perfusion model and method were used to determine the intestinal absorption sites and absorption characteristics of madecassoside and the effects of different surfactants on intestinal absorption. RESULTS: Madecassoside absorbed stronger in the small intestine of rats than in the large intestine and its rate of absorption was ileum> jejunum ≥ duodenum> colon. However, there was no significant difference in ileal absorption rate and absorption rate constant between different concentrations of madecassoside solution. Among the three different surfactants, polysorbate-80 has a significant promoting effect on the ileal absorption of madecassoside. CONCLUSIONS: The intestinal absorption of asiaticoside mainly concentrates in the middle part of the small intestine, and the absorption mechanism is presumed to be passive diffusion. The surfactant polysorbate-80 can promote the ileal absorption.