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目的 探讨血管紧张素Ⅱ 1型受体 (angiotensinⅡ type 1receptor,AT 1R)基因多态性与妊娠高血压综合征 (简称妊高征 ,PIH)的相关性。方法 采用聚合酶链反应 限制性酶切片段长度多态性 (PCR RFLP)方法检测90例妊高征患者和 90例正常妊娠妇女的AT 1R基因型 ;同时以放免法测定其外周血的血管紧张素Ⅱ (angiotensinⅡ ,AngⅡ )水平。结果 两组均存在A116 6C、C5 73T的基因多态性 ,而在 87、186、10 82和 15 17核苷酸位点均未发现基因多态性。其中妊高征组C 116 6等位基因、AC/CC基因型的频率显著高于对照组 ;5 73位点T等位基因、TT基因型的频率两组无显著性差异 ;两组AngⅡ水平无显著性差异 ;Logistic回归分析显示AT 1RA1166→C变异基因是妊高征发生的独立相关变量。结论 AT 1RA1166位点变异 (A→C)与妊高征的发生相关 ,可作为妊高征发病易感性的标记。
Objective To investigate the association of angiotensin Ⅱ type 1 receptor (AT1R) gene polymorphism with pregnancy induced hypertension (PIH). Methods The genotypes of AT1R in 90 patients with PIH and 90 normal pregnant women were detected by polymerase chain reaction restriction fragment length polymorphism (PCR RFLP). At the same time, the levels of AT 1R in peripheral blood were determined by radioimmunoassay Angiotensin Ⅱ (AngⅡ) levels. Results The polymorphisms of A116 6C and C5 73T existed in both groups, while no gene polymorphism was found in 87, 186, 10 82 and 15 17 nucleotide sites. Among them, the frequency of C 116 6 allele and AC / CC genotype in PIH group was significantly higher than that in control group. There was no significant difference in frequency of TT genotype between 5 T and T allele 573 loci; No significant difference; Logistic regression analysis showed AT 1RA1166 → C variant gene is an independent variable related to the occurrence of pregnancy-induced hypertension. CONCLUSION: The site variation of AT 1RA1166 (A → C) is associated with the occurrence of PIH and can be used as a marker for the susceptibility to PIH.