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[目的]采用D-酪氨酸荧光猝灭法建立同时测定6-苄基腺嘌呤和糠氨基嘌呤的定量分析方法。[方法]利用荧光偏振信号计算获得的荧光偏振度(p)和荧光各向异性(r)来区分6-苄基腺嘌呤和糠氨基嘌呤这2种天然植物性激素,同时利用荧光猝灭信号和双标准曲线计量分析法同时测定这2种天然植物性激素。[结果]D-酪氨酸-6-苄基腺嘌呤体系对应着较大的荧光偏振度和荧光各向异性度,而D-酪氨酸-糠氨基嘌呤体系则对应着较小的荧光偏振度和荧光各向异性度,因此通过计算其荧光偏振度和荧光各向异性可对这2种细胞分裂素的混合体系进行研究与表征。[结论]该法可以应用于市售李子和苦瓜样品表皮中6-苄基腺嘌呤和糠氨基嘌呤的同时测定,结果令人满意。
[Objective] To establish a quantitative method for the simultaneous determination of 6-benzyladenine and furfurylpurine using D-tyrosine fluorescence quenching. [Method] Two kinds of natural phytohormones, 6-benzyl adenine and furfuryl purine, were distinguished by fluorescence polarization (p) and fluorescence anisotropy (r) calculated by fluorescence polarization signal. Meanwhile, fluorescence quenching signal and Double Standard Curve Metrology Analysis Simultaneous Determination of Two Natural Plant Hormones. [Result] The D-tyrosine-6-benzyladenine system corresponded to a large degree of fluorescence polarization and fluorescence anisotropy, whereas the D-tyrosine-furfuryl purine system corresponded to a smaller fluorescence polarization Degree and fluorescence anisotropy, so the two kinds of cytokinin mixed system can be studied and characterized by calculating the fluorescence polarization and fluorescence anisotropy. [Conclusion] The method could be applied to the simultaneous determination of 6-benzyladenine and furfuryl purine in the epidermis of plum and balsam pear samples with satisfactory results.