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用两步法从WuT3细胞培养上清中提取WuT3单克隆抗体（McAb），首先将培养上清直接上SP离子交换柱，再上DEAE柱，获取提纯的McAb。该工艺的确定及放大均在Pharmacia产Biopilot中试纯化系统上进行。一次投料20L，纯化规模达2g，纯化的McAb经SDS－PAGE测定，纯度基本达到95％，免疫荧光活性为66±9％。热原质合格，支原体、残余DNA均为阴性，达到体内制剂质控要求。
WuT3 monoclonal antibody (McAb) was extracted from WuT3 cell culture supernatant by two-step method. First, the culture supernatant was directly applied to SP ion exchange column and then to DEAE column to obtain purified McAb. The process of determination and amplification are Pharmacia production Biopilot pilot purification system. Once fed 20L, the purification scale up to 2g, purified McAb by SDS-PAGE assay, the purity was basically 95%, immunofluorescence activity of 66 ± 9%. Qualified pyrogen, Mycoplasma, residual DNA were negative, to achieve the quality requirements of the preparation of the body.