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采用最新合成的荧光试剂与大白鼠组织及食物中α-二羧基类化合物生成荧光诱导体，然后用反相HPLC－荧光法对大白鼠组织及食物中的乙二醛、甲基乙二醛、二乙酸和2，3－乙酸丙酮4种α-二羧基类化合物同时进行分离定量检出，色谱柱为L－ODS（100×8mmid），流动相为乙膊-0．5mol／L醋酸铵（35：65V／V），荧光检出波长为Ex350nmEm390nm，检出限度（S／N－3）11．6～13．8fmol／10ul，回收率均在90％以上。
The fluorescence inducer was produced by using the newly synthesized fluorescent reagent and α-dicarboxy compounds in the tissues and foods of rats. Then, the content of glyoxal, methylglyoxal, Diacetic acid and 2,3-acetic acid acetone four kinds of α-dicarboxy compounds were simultaneously detected quantitatively, the column for the L-ODS (100 × 8mmid), the mobile phase is B-0.5mol / L ammonium acetate ( 35: 65V / V). The fluorescence detection wavelength was Ex350nmEm390nm, the detection limit (S / N-3) was 11.6-13.8fmol / 10ul, and the recoveries were above 90%.