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目的:通过观察王氏保赤丸对小鼠肠道菌群中双歧杆菌、乳酸杆菌、肠球菌和大肠埃希菌数量的影响,从肠道菌群变化方面研究长期用王氏保赤丸是否引起小鼠肠道菌群失调,为合理使用该药提供实验依据。方法:将实验小鼠分为王氏保赤丸高、中、低剂量组,分别以王氏保赤丸147、49、17mg/(kg.d)灌胃14天;设正常对照组和抗生素致菌群失调对照组,分别予生理盐水灌胃、盐酸林可霉素灌胃14天。取盲肠内容物培养后分离双歧杆菌、肠球菌、乳酸杆菌、大肠杆菌,鉴定并计数。结果:各组大肠杆菌数量无差异。抗生素组肠球菌数量增加、乳酸杆菌和双歧杆菌数量减少;王氏保赤丸高、中、低剂组3种菌群计数无明显变化,与抗生素组比较有明显差异(P<0.05),与正常组比较无显著差异。结论:长期服用王氏保赤丸不会引起抗生素样菌群失调。
OBJECTIVE: To investigate the effect of Wangshi Baochi Wan on the quantity of bifidobacteria, lactobacilli, enterococci and Escherichia coli in intestinal flora in mice and to study whether long-term Wangshi Baochi Wan caused by changes in intestinal microflora Mice intestinal flora imbalance, to provide experimental evidence for the rational use of the drug. Methods: The experimental mice were divided into high, medium and low dose groups of Wang Baochi Pills, respectively, with Wang Baoshi Red Pills 147,49,17 mg / (kg.d) for 14 days; normal control group and antibiotic-induced bacteria Group control group were given normal saline gavage, lincomycin hydrochloride intragastric administration for 14 days. The contents of cecum were isolated after bifidobacteria, enterococci, lactobacillus, E. coli, identified and counted. Results: There was no difference in the number of Escherichia coli in each group. The number of enterococci in antibiotic group increased, while the number of Lactobacillus and Bifidobacterium decreased. There was no significant change in the counts of three kinds of bacteria in high, middle and low doses of Wangshi Baochi Pills (P <0.05) No significant difference between normal group. Conclusion: Long-term use of Wang Baochi pills does not cause antibiotic-like flora.