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DNA含量与染色体倍体数有着直线相关关系。通过流式细胞分析术(FCM)细胞DNA含量分析,可能为前列腺癌的预后提供一种有用的信息。作者选择80例病理证实的前列腺癌病人进行FCM,就DNA含量与肿瘤分期、分级以及病人的存活关系进行评价。这些病人均用LHRH类似物或联用甲基孕酮治疗,根据Mostofi方法进行组织学分级。随访54～102个月,并用Kaplan-Meier方法标出生存曲线,80例病人在超声引导下经会阴行前列腺穿刺活检;标本放人含5%胎牛血清的RPMI1640培养基
The DNA content has a linear relationship with the ploidy number. Analysis of cellular DNA content by flow cytometry (FCM) may provide a useful information for the prognosis of prostate cancer. The authors selected 80 patients with pathologically proven prostate cancer to undergo FCM and evaluated the relationship between DNA content and tumor stage, grade, and patient survival. These patients were treated with LHRH analogues or in combination with methyl progesterone and histologically graded according to the Mostofi method. Follow-up was 54 to 102 months. The survival curve was marked by the Kaplan-Meier method. 80 patients underwent ultrasound-guided prostate biopsy through the perineum; specimens were given RPMI 1640 medium containing 5% fetal bovine serum.