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A simple and novel method was developed to determine methylene blue(MB) by resonance light scattering(RLS) using silica nanoparticles(SiO_2NPs) as the probe.It was found that MB could enhance the RLS intensity of SiO_2NPs.Moreover,the increase in RLS intensity was linear with the concentration of MB over the range of 0.01-3.0 μg mL~(-1).The limit of detection(LOD) was as low as 4.36 ng mL~(-1)(3σ) and the relative standard deviation(RSD) was 2.4%(n=6).Under the optimum experimental conditions,this proposed method was successfully applied for the determination of MB in aquaculture samples with recoveries between 96.3% and 107%.Possible mechanisms for the RLS enhancement of SiO_2NPs in the presence of MB were also discussed.
A simple and novel method was developed to determine methylene blue (MB) by resonance light scattering (RLS) using silica nanoparticles (SiO 2 NPs) as the probe. It was found that MB could enhance the RLS intensity of SiO 2 NPs. More over, the increase in RLS intensity was linear with the concentration of MB over the range of 0.01-3.0 μg mL -1. The limit of detection (LOD) was as low as 4.36 ng mL -1 (3σ) and the relative standard deviation (RSD) was 2.4% (n = 6) .Under the optimum experimental conditions, this proposed method was successfully applied for the determination of MB in aquaculture samples with recoveries between 96.3% and 107% .Possible mechanisms for the RLS enhancement of SiO 2 NPs in the presence of MB were also discussed.