论文部分内容阅读
目的:比较不同剂量亚氨基二丙腈(IDPN)对诱导的多发性抽动症(TS)大鼠模型的影响。方法:80只雄性SD大鼠随机分为空白对照组、低剂量组、中剂量组、高剂量组并分别予生理盐水、IDPN 150 mg/kg、IDPN300 mg/kg、IDPN 350 mg/kg腹腔注射,每日1次,连续7日,比较各组大鼠一般情况、刻板行为、运动行为、病理学形态及多巴胺(DA)含量变化。结果:较空白对照组,低剂量组一般情况好,未出现死亡,刻板行为、运动行为增多,病理学改变不明显,DA含量变化不明显;中剂量组一般情况好,死亡率低,刻板行为、运动行为明显增多,病理学形态改变不明显,DA含量变化不明显;高剂量组一般情况较差,死亡率高,刻板行为、运动行为显著增多,病理学形态改变明显,DA含量未见明显变化。结论:本实验成功建立了TS大鼠模型,三种剂量均可完成造模,中剂量组(300 mg/kg)可缩短造模时间,成功率高,死亡率低,能为TS的实验研究提供较好的可复制动物模型。
Objective: To compare the effects of different doses of iminodipropiononitrile (IDPN) on induced rat models of multiple tic syndrome (TS). Methods: Eighty male Sprague-Dawley rats were randomly divided into blank control group, low dose group, middle dose group and high dose group and received normal saline (IDPN 150 mg / kg, IDPN 300 mg / kg, IDPN 350 mg / , Once daily for 7 consecutive days. The general condition, stereotyped behavior, motor behavior, pathological morphology and dopamine (DA) content of rats in each group were compared. Results: Compared with the blank control group, the low dose group was generally good, no death occurred, the stereotyped behavior and motor behavior increased, the pathological changes were not obvious, the changes of DA content were not obvious. The middle dose group was generally good, the mortality rate was low, , There was a significant increase in motor activity, with no significant change in pathological morphology and no significant change in DA content. The high-dose group was generally poor, with high mortality, stereotypic behavior and motor activity, significant changes in pathological morphology, and no significant changes in DA content Variety. CONCLUSIONS: TS rat model was successfully established in this experiment, and all three doses can be used for modeling. The middle dose group (300 mg / kg) can shorten the modeling time, high success rate and low mortality rate, which can be the experimental study of TS Provide better replicable animal models.