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目的:探讨蛋白酶体抑制剂3,4-二氯异香豆素(DCI)对人胃癌细胞系BGC823体外增殖和凋亡的影响。方法:将DCI加入BGC823胃癌细胞,采用MTT法检测细胞的生长抑制效应,AnnexinⅤ/PI法及电镜观察DCI对细胞凋亡的诱导作用。结果:DCI可明显抑制BGC823细胞体外增殖,随着DIC浓度的增加和处理时间的延长,细胞凋亡率增加;流式细胞术显示DCI可诱导胃癌细胞早期凋亡,细胞被阻滞在G1期;电镜观察发现,经DCI处理后的部分细胞具有凋亡细胞的典型形态特征。结论:DCI可抑制人胃癌BGC823细胞增殖并促进其凋亡。
AIM: To investigate the effects of proteasome inhibitor 3,4-dichloroisocoumarin (DCI) on the proliferation and apoptosis of human gastric cancer cell line BGC823 in vitro. Methods: The DCI was added into BGC823 gastric cancer cells. The growth inhibition effect was detected by MTT assay. The apoptosis induced by DCI was observed by AnnexinⅤ / PI and electron microscopy. RESULTS: DCI significantly inhibited the proliferation of BGC823 cells in vitro. The apoptosis rate of BGC823 cells was increased with the increase of DIC concentration and the prolongation of treatment time. Flow cytometry showed that DCI induced the early apoptosis of gastric cancer cells. The cells were arrested in G1 phase Electron microscopy showed that some cells treated with DCI had typical morphological features of apoptotic cells. Conclusion: DCI can inhibit the proliferation of human gastric cancer BGC823 cells and promote their apoptosis.