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目的：：研究羽扇豆醇( Lupeol)在人高转移肝癌细胞系HCCLM3中的抗增殖作用机制。方法：运用CCK-8法检测不同浓度Lupeol在12-48 h对HCCLM3细胞活力的影响及相关Caspase参与Lupeol(20~100μmol·L-1)诱导的细胞凋亡类型；运用Realtime PCR评价Lupeol对细胞内Caspase家族及Bcl-2相关基因的mRNA表达的影响；同时运用流式细胞术检测Lupe-ol对细胞周期分布的影响。结果：Lupeol在24~48 h能够抑制HCCLM3的细胞增殖，并呈现浓度依赖性，在24 h处理细胞的IC50为93μmol·L-1；运用60~100μmol·L-1的Lupeol能够使HCCLM3细胞在G2/M期细胞数增加1倍；Lupeol能够活化Caspase通路，与对照组相比Lupeol处理后细胞内Caspase-3的mRNA表达量增加50%~150%，同时100μmol·L-1的Lupeol处理细胞后使胞内p53及Bax的mRNA表达量分别上调1倍以上，并显著降低Bcl-2及PARP的mRNA水平( P<0.05或P<0.01)。结论：Lupeol具有抑制肝癌细胞增殖能力，对肝癌预防及治疗可能具有一定的协同作用。“,”Objective:To study the mechanism of anti-proliferative effect of lupeol on highly metastatic human hepatocellular car-cinoma HCCLM3 cells. Methods:CCK-8 assay was performed to evaluate the effects of lupeol at different concentration on cell viability in 12-48 h. Caspase inhibitors were used to identify the subtypes of caspases activated during lupeol-induced cell death. The effects of lupeol on the mRNA expression of caspase family and Bcl-2 related genes were detected by real-time PCR. The effects of lupeol on HC-CLM3 cell phase distribution were investigated by flow cytometry. Results:Compared with the control group, lupeol could inhibit HC-CLM3 cell proliferation in a concentration-dependent manner with IC50 of 93 μmol·L-1 in 24h. The number of HCCLM3 cells in the period of G2/M was increased by 1-fold when the lupeol concentration was within 60-100 μmol·L-1 . Lupeol could activate the path-way of caspase, and the mRNA expression of caspase-3 was elevated by 50%-150% when compared with that in the control group. Mo-reover, the mRNA expression of p53 and Bax were increased above 1-fold by lupeol at 100 μmol·L-1 , and the Bcl-2 and PARP ex-pression were significantly suppressed by lupeol at 60-100 μmol·L-1(P<0. 05 or P<0. 01). Conclusion:The results indicate that lupeol has anti-proliferative effect on the liver cancer cells, which is beneficial to the prevention and treatment of liver cancer.