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三、快速繁殖的基本生产阶段 1974年,Murashige指出,通过组织培养繁殖植物需要经过三个步骤,即阶段Ⅰ、阶段Ⅱ和阶段Ⅲ。1981年Debergh等人建议增加一个阶段0,把阶段Ⅲ分开成为阶段Ⅲ_a和Ⅲ_b两个阶段。1984年,George等人建议把阶段Ⅲ_b单独列为阶段Ⅳ。这样,花卉园艺植物的试管快速繁殖步骤就由阶段0到阶段Ⅳ五个顺序步骤构成。尽管上述五个阶段的顺序步骤并不是每个步骤都是必需的,但是,必须对相应步骤的每个阶段建立适宜条件。
Third, the basic stage of rapid breeding In 1974, Murashige pointed out that through tissue culture and propagation of plants need to go through three steps, namely stage Ⅰ, stage Ⅱ and stage Ⅲ. In 1981 Debergh et al proposed to add a stage 0, the stage Ⅲ divided into stages Ⅲ_a and Ⅲ_b two stages. In 1984, George et al. Suggested that stage III_b should be individually classified as stage IV. In this way, flower horticultural plant tube rapid propagation step from stage 0 to stage IV five sequential steps. Although the sequential steps of the above five phases are not necessary for every step, suitable conditions must be established for each phase of the corresponding step.