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目的调查新疆部分地区人群及动物感染恙虫病东方体(Ot)情况,并对媒介昆虫进行调查。方法采用多种方法捕获可能感染Ot动物取脾,当地牧民静脉取血,从野鼠体表捕获恙螨,用试剂盒提取DNA,应用巢式PCR(nPCR)检测Ot-Sta56基因;接种昆明小白鼠进行Ot病原传代分离。结果共采取人群血液2430份,在6份血液中检测并分离到Ot;捕获动物13种5783个(只),从5种啮齿动物(小家鼠、灰仓鼠、大砂土鼠、草原兔尾鼠、灰旱獭)、2种鸟类(麻雀、灰腹鹡鸰)的脾中检测并分离到Ot,从绵羊血液中检测到Ot。其他动物体内未检测到Ot。从野鼠体表分离恙螨5种,从博乐纤恙螨体内检测分离到Ot。基因序列分析表明,新疆地区存在恙虫病东方体的基因型以Karp型为主,可能存在Saitama型。结论新疆地区存在人群及动物感染Ot,其基因型以Karp为主,可能存在Saitama型。
Objective To investigate the prevalence of Ot in tsutsugamushi disease in some areas of Xinjiang and investigate the vector insects. Methods A number of methods were used to capture the animals that may be infected with Ot and the venous blood of local herdsmen was collected to capture the chigger mites. The DNA was extracted with the kit, and the Ot-Sta56 gene was detected by nested PCR (nPCR) Ot pathogen transmission separation. Results A total of 2430 blood samples were collected from 6 blood samples and Ot was detected in 6 blood samples. A total of 5783 animals were collected from 13 rodents (Mus musculus, gray hamster, Mouse, gray marmot), Ot in the spleen of two species of birds (sparrow, gray-bellied grub) and Ot from sheep blood. Ot was not detected in other animals. Five species of chigger mites were isolated from the surface of wild rodents, and Ot was detected from the body of Bochi chigger mites. Genetic sequence analysis showed that the genotype of Orientia tsutsugamushi in Xinjiang was Karp-type and Saitama-type probably existed. Conclusion In Xinjiang, Ot exists in populations and animals, and its genotype is predominately Karp. There may be Saitama type.