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目的采用UPLC法同时测定通天口服液中没食子酸、天麻素和芍药苷的含量。方法采用Zorbax Eclipse Plus C_(18)柱(100 mm×2.1 mm,1.8μm),流动相为乙腈-0.05%磷酸溶液,梯度洗脱,流速0.3 m L·min~(-1),检测波长230 nm,柱温25℃。结果没食子酸、天麻素和芍药苷的线性范围分别为23.84~238.4、9.552~95.52、33.56~335.6 ng;平均加样回收率分别为100.2%、101.0%、101.0%,RSD分别为1.6%、1.8%、1.5%(n=6)。结论所用方法简便、快速、准确,可为通天口服液的质量控制提供参考。
Objective To determine the content of gallic acid, gastrodin and paeoniflorin in Tongtian oral liquid by UPLC method. Methods The mobile phase consisted of acetonitrile - 0.05% phosphoric acid solution with gradient elution at a flow rate of 0.3 mL · min -1 on a Zorbax Eclipse Plus C_ (18) column (100 mm × 2.1 mm, 1.8 μm) nm, column temperature 25 ℃. Results The linear ranges of gallic acid, gastrodin and paeoniflorin were 23.84 ~ 238.4, 9.552 ~ 95.52 and 33.56 ~ 335.6 ng, respectively. The average recoveries were 100.2%, 101.0% and 101.0%, respectively, and the RSDs were 1.6% and 1.8% %, 1.5% (n = 6). Conclusion The method is simple, rapid and accurate, which can provide reference for the quality control of Tongtian oral liquid.