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传统的基于纳米金的侧流免疫层析法灵敏度不高。为了克服这个缺点并且不增加额外的步骤,该研究通过双纳米金标记技术结合生物素-链霉亲和素蛋白放大系统,并利用金标定量阅读仪达到快速定量检测骨代谢标志物(以骨钙素为例)的目的。该试纸条的第一结合垫包被链霉亲和素-纳米金探针,第二结合垫包被抗骨钙素单抗Ab14318-纳米金-生物素化单链核苷酸探针,硝酸纤维素膜上的检测线包被抗骨钙素单抗Ab14319,质控线上包被羊抗鼠Ig G。通过对该侧流免疫层析试纸条与电化学免疫测定法(ECLIA)的相关性研究发现,两种方法有强相关性。该研究表明该试纸条拥有良好灵敏度、准确度和可靠性。与电化学免疫测定法相比,该方法更加简单、快速,并且不需要复杂的仪器设备,非常适合在检测现场或基层社区医院使用,应用前景非常广泛。
The traditional gold nanoparticle-based lateral flow immunochromatography is less sensitive. In order to overcome this disadvantage and without adding extra steps, this study combined biotin-streptavidin amplification system with dual-gold nanoparticle labeling and achieved rapid and quantitative detection of bone metabolism markers Calcium for example) purposes. The first binding pad of the test strip is coated with streptavidin-nano gold probe, the second binding pad is coated with anti-osteocalcin monoclonal antibody Ab14318-nano gold-biotinylated single-stranded nucleotide probe, The detection line on the nitrocellulose membrane was coated with anti-osteocalcin monoclonal antibody Ab14319 and the control line coated goat anti-mouse IgG. The correlation between the lateral flow immunoassay strip and electrochemical immunoassay (ECLIA) was studied and found to be strongly correlated. The study shows that the strip has good sensitivity, accuracy and reliability. Compared with electrochemical immunoassay, the method is simple, rapid and does not require complicated equipment. It is very suitable for use in the detection site or grass-roots community hospitals, and has a wide range of applications.