论文部分内容阅读
目的制备淫羊藿苷元自微乳,考察其理化性质及体外肠吸收特性。方法在考察淫羊藿苷元理化性质的基础上,以油酸乙酯为油相,聚山梨酯80为乳化剂,丙三醇为助乳化剂制备淫羊藿苷元自微乳,采用透射电镜及激光粒度分析仪测定其稀释后所得微乳的形态、粒径分布和Zeta电位,并采用Caco-2细胞模型初步分析其肠吸收特性。结果所制备的淫羊藿苷元自微乳稀释后,微乳平均粒径为55.6 nm,Zeta电位为30.8 mV,在Caco-2细胞模型上的表观渗透系数(Papp)为(3.52±0.3)×10 6cm/s。结论淫羊藿苷元自微乳制剂稳定,体外研究显示自微乳系统能够促进淫羊藿苷元在肠道的吸收。
Objective To prepare icariin self-microemulsions and study its physicochemical properties and intestinal absorption characteristics. Methods Based on the study of physical and chemical properties of icariin, oleic acid ethyl ester as oil phase, polysorbate 80 as emulsifier and glycerol as co-emulsifier to prepare icariin self-microemulsion, The morphology, particle size distribution and Zeta potential of the microemulsion obtained after dilution were measured by electron microscope and laser particle size analyzer. The intestinal absorption characteristics were analyzed by Caco-2 cell model. Results After the icariin was diluted by microemulsion, the average particle size of the microemulsion was 55.6 nm and the Zeta potential was 30.8 mV. The apparent permeability coefficient (Papp) of the icaritin in the Caco-2 cell model was (3.52 ± 0.3) ) × 10 6 cm / s. Conclusion The icariin self-microemulsion preparation is stable. In vitro studies show that the self-microemulsion system can promote the absorption of icariin in the intestine.