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用半导体激光(3.97 mW/mm2)和相干光及非相干光转换器(专利:CN2555523Y)将半导体激光转换为同功率、同波长和同光斑大小的非相干红光辐照小麦种子.通过分析幼苗期叶片DNA及生理变化,考察低剂量激光防护紫外线-B(UV-B)辐射损伤小麦幼苗的光效应.半导体激光预处理使10.08 kJ/m2UV-B辐射损伤小麦幼苗超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,紫外吸收物含量、可溶性蛋白质含量、叶绿素a和叶绿素b含量及根长显著增加,丙二醛(MDA)含量显著降低.而非相干红光则不能.用酶联免疫(ELISA)方法检测小麦叶片DNA受UV-B辐射损伤产生的环丁烷嘧啶二聚体(CPD)含量,发现半导体激光能显著降低UV-B辐射损伤小麦细胞DNA中环丁烷嘧啶二聚体的含量,而非相干红光却不能使其降低.说明半导体激光防护UV-B辐射损伤小麦幼苗中光效应不起作用.
Semiconductor laser (3.97 mW / mm2) and coherent light and incoherent light converter (patent CN2555523Y) were used to convert the semiconductor laser into wheat with the same power and the same wavelength and spot size incoherent red light.Through the analysis of seedlings The effects of low dose UV-B irradiation on wheat seedlings were investigated by DNA pretreatment and laser irradiation. Semiconductor laser pretreatment led to the decrease of superoxide dismutase (SOD) in wheat seedlings under 10.08 kJ / m2 UV-B radiation POD, CAT activity, UV absorbance, soluble protein content, chlorophyll a and chlorophyll b content and root length increased significantly, but malondialdehyde (MDA) content decreased significantly. But non-coherent red light could not.Using enzyme-linked immunosorbent assay (ELISA) to detect the content of cyclobutane pyrimidine dimer (CPD) produced by UV-B radiation in wheat leaf DNA, we found that the semiconductor laser can significantly reduce the UV-B radiation Damage to wheat DNA content of cyclobutane pyrimidine dimer, but non-coherent red light can not make it lower, indicating that the semiconductor laser protection UV-B radiation damage in wheat seedling light effect does not work.