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目的采用LC-MS/MS考察α-倒捻子素脂质体的药动学特征。方法采用SPF级ICR小鼠,♀,尾静脉注射α-倒捻子素脂质体3 mg·kg-1,用LC-MS/MS测定α-倒捻子素血药浓度,并计算其药动学参数。结果α-倒捻子素线性范围为5~1 000 ng·m L~(-1)(r=0.997 6),定量限为5 ng·m L~(-1),日内、日间精密度均<11.0%。主要药动学参数:t1/2为(0.99±0.12)h,Cmax为(1 524.60±323.82)ng·m L~(-1),AUC0-t为(645.84±63.74)ng·h·m L~(-1),MRT为(0.70±0.11)h,CL为(4.80±0.45)m L·h~(-1)·kg~(-1),Vd为(6.80±1.00)m L·kg~(-1)。结论该法操作简便、快速、灵敏,可用于测定小鼠血浆中α-倒捻子素浓度。本研究结果可为药物的进一步研究提供依据。
Objective To investigate the pharmacokinetics of α-mangostin liposomes by LC-MS / MS. Methods The SPF-ICR mice were injected intravenously with 倒 and tail vein. The plasma concentration of α-mangostin was determined by LC-MS / MS. The pharmacokinetics parameter. Results The linear range of α-mangostin was 5-1 000 ng · m L -1 (r = 0.997 6) and the limit of quantification was 5 ng · m L -1. The intra- and inter-day precision <11.0%. The main pharmacokinetic parameters were as follows: t1 / 2 was (0.99 ± 0.12) h, Cmax was (1 524.60 ± 323.82) ng · m L -1 and AUC0-t was (645.84 ± 63.74) ng · h · m L ~ (-1), MRT was (0.70 ± 0.11) h, CL was (4.80 ± 0.45) m L · h -1 · kg -1, Vd was (6.80 ± 1.00) m L · kg ~ (-1). Conclusion The method is simple, rapid and sensitive and can be used to determine the concentration of α-mangostin in mouse plasma. The results of this study provide a basis for further study of drugs.