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四氧化三铁纳米颗粒(Fe3O4-NPs)在生物医学领域有着广泛的应用。通过同步辐射真空紫外圆二色谱(SRCD)和紫外可见(UV-Vis)吸收谱技术研究了Fe3O4纳米颗粒的粒径(10 nm和40 nm)对细胞色素C(Cyt c)结构的影响。结果表明,随着Fe3O4-NPs颗粒物浓度的增大,Cyt c在408 nm处的吸光度值逐渐下降。SRCD结果显示Cyt c与Fe3O4-NPs作用后,蛋白的α螺旋含量减少,β折叠含量增加;Fe3O4-NPs与Cyt C的作用强度具有尺寸依赖性,小尺寸的Fe3O4-NPs作用更为明显。
Ferroferric oxide nanoparticles (Fe3O4-NPs) have a wide range of applications in the biomedical field. The effect of Fe3O4 nanoparticle size (10 nm and 40 nm) on Cyt c structure was investigated by synchronous vacuum-coupled ultra violet dichroism spectroscopy (SRCD) and UV-Vis absorption spectroscopy. The results showed that with the increase of the concentration of Fe3O4-NPs particles, the absorbance value of Cyt c at 408 nm decreased gradually. The results of SRCD showed that after treated with Cyt c and Fe3O4-NPs, the α-helix content and the content of β-sheet increased. The effect of Fe3O4-NPs and Cyt C was size-dependent, and the effect of small size Fe3O4-NPs was more obvious.