论文部分内容阅读
利用RAPD技术对小麦（黑麦）异源重组系异源2号组配的22个强优势杂交组合的亲本进行了遗传差异检测分析。结果表明，被测材料间RAPD标记多态性较高.61个随机引物中，37个引物（占60．65％）扩增产物具多态性，共扩增得到181条带。其中，105条带具多态性，占58．01％。每个引物可扩增出1～7条多态性带，平均可扩增2.8条多态性带。父本异源2号与其强优势组合母本间RAPD遗传距离较大，平均为0．43，聚类结果也显示其单独聚为一类。由此证实，异源2号与母本间确实在分子水平上存在较大遗传差异。RAPD遗传距离与亲本各性状表型差异相关均不显著，与F1各性状杂种优势间除抽穗期外相关也均不显著。据此认为，难以直接利用RAPD遗传距离预测杂交组合的杂种优势。
RAPD technique was used to detect the genetic variation of 22 parents with strong heterosis in wheat (rye) heterologous recombination lines. The results showed that there was a high RAPD marker polymorphism among the tested materials. Of the 61 random primers, 37 primers (60.65%) amplified polymorphisms and amplified 181 bands. Among them, 105 were polymorphic, accounting for 58.01%. Each primer can amplify 1 to 7 polymorphic bands, an average of 2.8 polymorphic bands can be amplified. The RAPD genetic distance between the male parent Heterologous 2 and its strong dominant parent was larger, with an average of 0.43, and the clustering results also showed that they were clustered together into one category. This confirmed that there is indeed a large genetic difference between Heterologous 2 and the female parent at the molecular level. The RAPD genetic distance was not significantly related to the phenotypic differences of the traits in the parents, but not to the F1 heterosis except for the heading stage. Therefore, it is difficult to predict the heterosis of hybrid combinations by RAPD genetic distance directly.