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采用Hypersil-ODS2(4.6mm×250mm,5μm)色谱柱,乙腈-水(70∶30)流动相,242nm检测波长,建立了测定2-苯基-4-对氯苯基-1,5-苯并硫氮杂-α-(丁二酰亚胺基)-β-内酰胺的高效液相色谱法。该法线性范为0～0.1mg/mL,回归方程为A=1.63×108c-2.86×105,相关系数r=0.9994,相对标准偏差为0.33%(c=0.06mg/mL,n=5),检出限为0.08μg/mL。平均回收率在97.2%～102.4%之间。该法重现性好,灵敏度高,简单,快速,准确。
The mobile phase of Hypersil-ODS2 (4.6 mm × 250 mm, 5 μm), mobile phase of acetonitrile-water (70:30) and detection wavelength of 242 nm were used for the determination of 2-phenyl-4-p-chlorophenyl- And high-performance liquid chromatography of thiazepine-α- (succinimidyl) -β-lactam. The regression equation was A = 1.63 × 108c-2.86 × 105, the correlation coefficient was 0.9994, the relative standard deviation was 0.33% (c = 0.06mg / mL, n = 5) The detection limit was 0.08μg / mL. The average recovery was between 97.2% and 102.4%. The law reproducible, high sensitivity, simple, fast and accurate.