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胃癌的早期诊断和治疗是提高胃癌患者预后的关键,纳米技术和干细胞是继手术、化疗和放疗之外新兴的肿瘤治疗新技术.制备了氨基化修饰的荧光磁性纳米粒子(FMNPs),无滋养层培养了小鼠胚胎干细胞(mESCs),体外实验评价了mESCs的条件培养基对胃癌细胞的影响,并利用FMNPs标记mESCs制备了干细胞纳米探针(FMNPs-mESCs),通过尾静脉注射进入荷瘤小鼠的体内,利用小动物活体成像系统考察了FMNPs-mESCs靶向识别体内胃癌细胞的能力.结果显示,制备的FMNPs具有良好的荧光性能和磁响应性能,在50μg/mL的浓度范围内对mESCs具有良好的生物活性,mESCs的条件培养基对胃癌细胞具有显著的抑制增殖的作用,FMNPs标记的mESCs具有良好的荧光性能,在注射进入荷瘤小鼠体内6h后可以通过活体成像结果和离体器官的荧光成像结果观察到肿瘤组织有显著的荧光信号.研究表明,FMNPs-mESCs具有特异靶向识别体内胃癌细胞的能力,为利用胚胎干细胞治疗胃癌提出新的研究方向.
The early diagnosis and treatment of gastric cancer is the key to improve the prognosis of patients with gastric cancer.Nano-technology and stem cells are new technologies in cancer treatment after surgery, chemotherapy and radiotherapy.Amino-modified fluorescent magnetic nanoparticles (FMNPs) Mouse embryonic stem cells (mESCs) were cultured in vitro. The effect of conditioned media of mESCs on gastric cancer cells was evaluated in vitro. Stem cell nano-probes (FMNPs-mESCs) were prepared by FMNPs labeled with mESCs. Mice were used to investigate the ability of targeting FMNPs-mESCs to target gastric cancer cells in vivo using small animal biopsy system.The results showed that the FMNPs prepared had good fluorescence and magnetic response properties at a concentration of 50μg / mL mESCs have good biological activity. The conditioned medium of mESCs can significantly inhibit the proliferation of gastric cancer cells. The FMNPs labeled mESCs have good fluorescence properties. After injection into tumor-bearing mice for 6h, Fluorescent imaging of body organs showed significant fluorescence signals in tumor tissue. Studies have shown that FMNPs-mESCs have a specific target Gastric cancer cells in vivo ability respectively, for the treatment of gastric Embryonic stem cells proposed new research directions.