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文中介绍扁平形、长方形和圆形横截面的细胞电泳小室中静止层位置的计算方法，指出对于正方形横截面说来，由壁面起算的静止层深度与小室高度之比应为△≈１/８，某些产品中通用的△－１／４是错误的。现有装置中静止层附近介质电渗速度变化较快，因而显微镜聚焦深度定位不准对于细胞电泳速率测量结果影响较大。作者建议设计双管式电泳仪，可使静止层附近的电渗速度始终保持几乎为零，从而大大减小深度定位不准所造成的测量误差。
In this paper, the method to calculate the position of the quiescent layer in cell electrophoresis chamber with flat, rectangular and circular cross section is introduced. The ratio of the depth of quiescent layer to the cell height for the square cross section is △ ≈1 / 8 , △ -1 / 4 common in some products is wrong. The electrodialysis velocity of the medium near the static layer in the existing device changes rapidly, therefore, the inaccurate positioning of the microscope focusing depth has a great influence on the cell electrophoresis velocity measurement result. The authors recommend designing a dual tube electrophoresis system that keeps electroosmotic velocities near the static layer nearly zero, greatly reducing the measurement error caused by inaccurate depth positioning.