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由白粉病菌(Blumeria graminisf.sp.tritici)引起的小麦白粉病是严重影响小麦安全生产的主要病害之一。本研究将来自以色列的野生二粒小麦(Triticum dicoccoides)WE27的坏白粉病基因通过杂交和连续回交,导入普通小麦遗传背景中,育成高抗白粉病小麦新品系3D256(其系谱为燕大1817/WE27//农大015/3/941,F6)。将3D256和高感小麦白粉病的普通小麦品系薛早配制杂交组合,对其F1、F2分离群体和F3家系进行白粉病抗性鉴定和遗传分析。结果表明,3D256携带抗白粉病显性单基因,暂命名为MlWE27。利用集群分离分析法(BSA)和分子标记分析,发现3个SSR标记(Xwmc243、Xwmc154和Xbarc318)、1个EST-SSR标记(Xdp357)、1个AFLP转化的SCAR标记(XCAUG1)和1个RFLP探针转化的STS标记(XWG516-1)与抗白粉病基因MlWE27连锁,在连锁图上的顺序为Xdp357-MlWE27-XCAUG1-XWG516-1-Xwmc243-Xwmc154-Xbarc318。利用中国春缺体-四体系、双端体系和缺失系将抗白粉病基因MlWE27定位于染色体2B短臂的末端Bin0.84-1.00上。这一普通小麦抗白粉病种质资源的创制及其连锁分子标记的建立为小麦抗病基因分子标记辅助选择、基因积聚和分子育种提供了新的物质基础。
Wheat powdery mildew caused by Blumeria graminis sp. Tritici is one of the major diseases that seriously affect the safe production of wheat. In this study, the powdery mildew genes of Triticum dicoccoides WE27 from Israel were crossed and backcrossed continuously into the common wheat genetic background to form 3D256, a new wheat line with high resistance to powdery mildew (the pedigree was YanDa 1817 / WE27 // CNN 015/3/941, F6). The crossing combinations of 3D256 and wheat cultivars with high susceptibility to wheat powdery mildew were crossed with Xuezao to identify and genetic analysis of powdery mildew resistance in F1 and F2 segregating populations and F3 families. The results showed that 3D256 carries the dominant single gene of powdery mildew and is temporarily named MlWE27. Three SSR markers (Xwmc243, Xwmc154 and Xbarc318), one EST-SSR marker (Xdp357), one AFLP transformed SCAR marker (XCAUG1) and one RFLP were found by cluster analysis (BSA) and molecular marker analysis The probe-transformed STS marker (XWG516-1) is linked to the powdery mildew resistance gene MlWE27 in the sequence of Xdp357-MlWE27-XCAUG1-XWG516-1-Xwmc243-Xwmc154-Xbarc318 on the linkage map. The Chinese spring foxtail-tetrasystem, two-terminal and deletion lines were used to map the MlWE27 gene on the end of chromosome 2B short arm, Bin0.84-1.00. The establishment of common wheat powdery mildew resistance germplasm resources and establishment of linkage molecular markers provide a new material basis for molecular marker-assisted selection, gene accumulation and molecular breeding of wheat disease resistance genes.