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用ICR纯种雄性小鼠24只,分空白对照组、高压和高压氧三组。后两组置于同一动物加压舱内,而高压氧组动物又在其中的小套舱内。前者给予5 ATA空气,后者吸入5ATA纯氧,停留37分钟。动物出舱后,迅速断头取材,分别按电镜形态观察和电镜细胞化学要求处理。1)超微结构变化高压组只有少数线粒体有轻度变化,其他细胞器与空白对照组无明显差别。高压氧组神经元结构有不同程度变化,少数线粒体肿胀,约1/4线粒体的嵴消失,基质清亮呈空泡,还有少数树突和线粒体内有致密物质。2)电镜细胞化学酶活性变化高压组毛细血管内皮AKP和G-6-P及神经元内的LDH活性比空白对照组稍增强。高压氧组LDH活性比高压组增强。三组实验中,高压氧组毛细血管内皮细胞的AKP、G-6-P活性反应最强。
Twenty-four ICR male mice were divided into blank control group, high pressure group and high pressure oxygen group. The latter two groups were placed in the same animal pressurization tank, while the hyperbaric oxygenated animals were in the small one of them. The former gives 5 ATA air, which inhales 5ATA pure oxygen and stays for 37 minutes. Animals out of space, rapid decapitation drawn, respectively, according to electron microscopy morphology and electron microscopy cytochemical requirements. 1) ultrastructural changes in high-pressure group only a few mitochondrial mild changes in other organelles and the blank control group no significant difference. The structure of neurons in hyperbaric oxygen group changed in varying degrees. A few mitochondria were swollen, the ridges of ¼ mitochondria disappeared, the matrix was clear and vacuolated, and a few dendrites and dense material in mitochondria. 2) Electron microscopy changes of cytochemical enzyme activity High-pressure group capillary endothelial AKP and G-6-P and LDH activity within neurons than the blank control group slightly enhanced. Hyperbaric oxygen LDH activity than the high-pressure group increased. In the three experiments, the activity of AKP and G-6-P in capillary endothelial cells of hyperbaric oxygen group was the strongest.