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本文建立了测定血浆中左旋四氢巴马汀浓度的反相高效液相色谱法。采用国产ＹＷＧ－Ｃ（１８）填充柱，以甲醇－水（７０∶３０）为流动相，安定作内标，在紫外检测器２８１ｎｍ波长处进行监测，按内标法定量。血样用二氯甲烷提取。最低检测浓度为０．０６μｇ／ｍｌ，线性范围０．６２５～４０μｇ／ｍｌ，日内日间测定的ＲＳＤ小于６．９％。
In this paper, an RP-HPLC method for the determination of L-tetrahydropalmatine in plasma was established. A column packed with domestic YWG-C (18) was used. The mobile phase consisted of methanol-water (70:30) was used as the internal standard. The detector was monitored at the wavelength of 281nm by UV detector and quantified by internal standard method. Blood samples were extracted with dichloromethane. The lowest detection concentration was 0.06μg / ml, the linear range of 0.625 ~ 40μg / ml, intraday RSD determination of less than 6.9%.