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[目的]提高龙牙百合组培苗移栽成活率,为龙牙百合种苗工厂化生产提供理论支持和技术指导。[方法]应用311-A最优混合设计法设计试验方案,研究不同浓度NAA、IBA和IAA对龙牙百合生根、结鳞茎的影响,用Excel制图,最后应用Qbasic寻优,获取其最佳培养基。[结果]龙牙百合无菌芽结鳞茎的最佳方案为NAA 0.30 mg/L+IBA 0.24 mg/L+IAA 0.24 mg/L;生根最佳方案为NAA 0.36mg/L+IBA 0.30 mg/L+IAA 0.36 mg/L。综合考虑,龙牙百合生根、结鳞茎的最佳培养基为NAA 0.33 mg/L+IBA 0.27 mg/L+IAA0.30 mg/L。经过验证,在最佳方案下,龙牙百合无菌芽结鳞茎数和生根数分别为48.0个/处理和18.67条/株。[结论]优化了龙牙百合生根、结鳞茎的培养基。
[Objective] To improve the transplanting survival rate of tissue culture seedlings of Aralia Lily, and provide theoretical support and technical guidance for the industrialized production of Aralia Lily seedlings. [Method] The optimum mixed design method of 311-A was used to design the experimental scheme. The effects of different concentrations of NAA, IBA and IAA on the rooting and tubering of Aralodendron lily were studied, and the results were plotted by Excel. Finally, Qbasic optimization was used to obtain the best culture base. [Result] The best plan of germination bulbless bulb was NAA 0.30 mg / L + IBA 0.24 mg / L + IAA 0.24 mg / L. The optimal plan of rooting was NAA 0.36 mg / L + IBA 0.30 mg / L + IAA 0.36 mg / L. Taken together, the best medium for the rooting and tuberization of A. japonica was NAA 0.33 mg / L + IBA 0.27 mg / L + IAA 0.30 mg / L. The results showed that under the optimum conditions, the numbers of germinated bulbs and rooting of Aralia elata were 48.0 / treatment and 18.67 / plant respectively. [Conclusion] The medium for rooting and bulb-forming of Aral is optimized.