论文部分内容阅读
为了评估甲氧虫酰肼的抗性风险,在室内进行了棉铃虫对甲氧虫酰肼的抗药性选育和现实遗传力分析。采用甲氧虫酰肼对棉铃虫敏感品系进行抗性筛选,共饲养33代,汰选29代,与敏感品系相比,获得抗性指数为32.99倍的抗性品系。采用阈性状分析方法,研究棉铃虫对甲氧虫酰肼的抗性现实遗传力,并对不同致死率下的抗性发展速率进行预测。结果表明,整个29代筛选期间,棉铃虫对甲氧虫酰肼的抗性现实遗传力(h2)为0.0830,在筛选的前、中、后期,h2分别为0.0715、0.0768和0.0947。根据室内筛选结果,假设斜率为2.0(δp=0.5),抗性现实遗传力为实际筛选估计值的一半,当杀死率为80%和90%时,棉铃虫对甲氧虫酰肼的抗性增加10倍,分别需要34.48代和27.40代。田间条件下,由于等位基因频率变化、环境变异等因素的影响,抗性增加10倍则需要更长的时间。
In order to assess the resistance risk of methoxyfenozide, resistance breeding and realistic heritability analysis of cotton bollworm to methoxyfenozide were conducted indoors. The resistant strain of cotton bollworm was screened by methoxyfenozide for 33 passages and 29 passages, compared with the susceptible lines, the resistant lines with resistance index of 32.99 were obtained. The thresholding method was used to study the heritability of resistance to methoxyfenozide in cotton bollworm (Helicoverpa armigera), and the rate of resistance development at different lethality was predicted. The results showed that during the entire 29 generations of selection, the actual heritability (h2) of the resistance of cotton bollworm to methoxyfenozide was 0.0830. The h2 was 0.0715, 0.0768 and 0.0947, respectively, before, during and after screening. Based on the in-house screening results, assuming that the slope is 2.0 (δp = 0.5), the resistance hereditary heritability is half of the actual screening estimate. When the kill rate is 80% and 90%, the resistance of cotton bollworm to methoxyfenozide Sex increased by 10 times, respectively, 34.48 and 27.40 generations. Field conditions, due to changes in allele frequency, environmental variation and other factors, a 10-fold increase in resistance will take longer time.