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使用0.1—0.4％的琼脂制成浆状的培养基,在植物组织培养中有着良好的效果。该培养基有如下优点:①效果好。用该培养基繁殖试管苗因有液层,保湿效果好,有利于试管苗的生长。如菊花叶片愈伤组织在该培养基中培养40天可产生出10—20个芽,而固体培养基(琼脂为0.7—0.8%)只能产生出6—8个劳。②培养时间长,培养基利用率高。利用30毫升该培养基,在100毫升三角瓶内繁殖菊花试管苗,可连续培养80天,且培养基能充分利用,而固体培养基只能培养40天,且留下10
The use of 0.1-0.4% agar to make a slurry medium has good results in plant tissue culture. The medium has the following advantages: ① effective. With this medium propagation of plantlets due to the liquid layer, moisturizing effect, is conducive to the growth of test tube seedlings. If chrysanthemum leaf callus cultured in this medium for 40 days can produce 10-20 buds, solid medium (agar 0.7-0.8%) can only produce 6-8 workers. ② culture for a long time, medium high utilization rate. Using 30 ml of this medium, chrysanthemum plantlets were propagated in a 100 ml Erlenmeyer flask continuously for 80 days and the medium was fully utilized, whereas the solid medium was allowed to grow for only 40 days, leaving 10