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目的探讨胰岛素样生长因子-Ⅱ(insulin-likegrowthfactorⅡ,IGF-Ⅱ)与胰岛素样生长因子结合蛋白1(insulin-likegrowthfactorbindingprotein-1,IGFBP-1)在妊娠早期对胚胎发育的影响。方法应用逆转录聚合酶链反应(RT-PCR)、酶联免疫吸附试验(ELISA)分别检测2002-04-2004-01中山大学附属一院47例早孕空胚妊娠妇女(观察组)和38例正常早孕人流妇女(对照组)绒毛组织中的IGF-Ⅱ、蜕膜组织IGFBP-1的mRNA表达量,及母血清中IGFBP-1水平,比较两组数值的相关性。结果观察组蜕膜组织IGFBP-1mRNA为(3·30±0·32)mg/L,绒毛组织IGF-ⅡmRNA为(1·50±0·41)mg/L,母血清IGFBP-1为(50·87±12·08)μg/L;对照组蜕膜组织IGFBP-1mRNA为(2·14±0·21)mg/L,绒毛组织IGF-ⅡmRNA为(3·80±0·17)mg/L,母血清IGFBP-1为(24·31±3·61)μg/L。观察组与对照组间IGF-ⅡmRNA、IGFBP-1mRNA及母血清IGFBP-1比较差异有统计学意义(P<0·05);绒毛组织中IGF-ⅡmRNA与蜕膜组织中IGFBP-1mRNA的表达量呈负相关,蜕膜组织IGFBP-1mRNA与母血清IGFBP-1呈正相关。结论IGF-Ⅱ、IGFBP-1可能可以作为早期胚胎发育预测潜能的指标。
Objective To investigate the effects of insulin-like growth factor-Ⅱ (IGF-Ⅱ) and insulin-like growth factor binding protein-1 (IGFBP-1) on embryonic development in early pregnancy. Methods 47 pregnant women (observation group) and 38 pregnant women (control group) of empty embryos of early pregnancy in Zhongshan First Affiliated Hospital of Sun Yat-sen University from April 2002 to April 2004 were detected by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) IGF-Ⅱ in chorionic tissue, decidual IGFBP-1 mRNA expression and IGFBP-1 in maternal serum in normal early pregnancy women (control group), and the correlation between the two groups were compared. Results IGFBP-1 mRNA in decidua was (3.30 ± 0.32) mg / L in decidua, (1.50 ± 0.41) mg / L in decidua and (50 · 87 ± 12.08 μg / L, IGFBP-1mRNA in the control group was (2.14 ± 0.21) mg / L and IGF-Ⅱ mRNA in the chorion was (3.80 ± 0.17) mg / L, IGFBP-1 of maternal serum was (24.31 ± 3.61) μg / L. IGF-ⅡmRNA, IGFBP-1mRNA and maternal serum IGFBP-1 in the observation group and the control group were significantly different (P <0.05); The expression of IGFBP-1 mRNA in the villus tissue and the decidual tissue There was a negative correlation between IGFBP-1 mRNA in decidua and IGFBP-1 in maternal serum. Conclusion IGF-Ⅱ and IGFBP-1 may be potential predictors of early embryonic development.