论文部分内容阅读
目的:研究15批次神农香菊挥发油气相色谱-质谱联用技术(GC-MS)指纹图谱与体外抗氧化能力的关系,为揭示神农香菊抗氧化物质基础提供依据。方法:15批次神农香菊采用水蒸气蒸馏法提取挥发油,并以HP-5MS毛细管柱(50 m×0.2 mm×0.33μm),程序升温(初始温度为50℃,以5℃·min~(-1)升至145℃,然后再以2℃·min~(-1)升至165℃,最终用20℃·min~(-1)升至250℃,保持5 min)等GC-MS分析条件建立挥发油指纹图谱。对15批次神农香菊挥发油、2,6-二叔丁基-4-甲基苯酚(BHT)及维生素E的体外抗氧化能力进行研究,数据统计分析后,采用灰色关联度分析法研究神农香菊挥发油抗氧化谱效关系。结果:建立15个批次神农香菊挥发油的指纹图谱,确定了15个共有色谱峰,并对共有峰进行了化学成分鉴定;体外抗氧化试验表明清除1,1-二苯基-2-三硝基苯肼(DPPH)能力从强至弱的顺序为BHT、神农香菊挥发油、维生素E,且各个浓度的神农香菊挥发油都对DPPH有清除作用,随着浓度的增加清除率也增大。15个共有色谱峰与抗氧化活性均存在一定的关联度,关联度在0.68~0.79,其中各成分与抗氧化间的关联度大小顺序为甜没药萜醇氧化物、安息香酸苄酯、β-桉叶醇、桉油烯醇、甜没药醇、α-环氧化红没药烯、乙酸氧化芳樟醇酯、金合欢烯、棕榈酸、脱氢芳樟醇、蛇床烯、蛇床烯醇、1.3.3-薄荷属三烯、芳樟醇、p-伞花烃。结论:该文初步探讨了神农香菊挥发油指纹特征峰抗氧化药效的大小,为神农香菊药用物质基础研究、药效评价及质量标准制定提供了科学依据与参考。
OBJECTIVE: To study the relationship between 15 fingerprints of Shennongxiangju volatile oil by gas chromatography-mass spectrometry (GC-MS) and antioxidant capacity in vitro, and to provide a basis for revealing the antioxidant base of Shennongxiangju. Methods: Fifteen batches of Shenxiang Chrysanthemum were extracted by steam distillation. The volatile oil was extracted by HP-5MS capillary column (50 m × 0.2 mm × 0.33 μm) and the temperature was programmed (the initial temperature was 50 ℃, at 5 ℃ · min ~ -1) to 145 ° C and then to 165 ° C at 2 ° C · min -1 and finally to 250 ° C at 20 ° C -1 (min -1) for 5 min) Conditions to establish volatile oil fingerprinting. 15 batches Shennongxiangju essential oil, 2,6-di-tert-butyl-4-methylphenol (BHT) and vitamin E in vitro antioxidant capacity of the study, statistical analysis, the use of gray correlation analysis Shennong Antioxidant spectrum effect of volatile oil from. Results: Fingerprints of 15 volatile oil from Shennongxiangju were established and 15 common chromatographic peaks were identified. The common peaks were identified by chemical constituents. Antioxidant tests in vitro showed that 1,1-diphenyl-2-tris The order of strong to weak nitrophenylhydrazine (DPPH) was BHT, Shennongxiangju volatile oil and vitamin E, and the volatile oil of Shennongxiangju at various concentrations all scavenged DPPH, and the clearance rate increased with increasing concentration . There were some correlations between the 15 common chromatographic peaks and the antioxidant activity, with correlations ranging from 0.68 to 0.79. The correlations between the components and the antioxidant activity were as follows: ibuprofen, benzyl benzoate, β - Eucalyptol, Eucalyptol, Bisabolol, α-epoxidized myrrh, linalyl acetate, farnesene, palmitic acid, dehydrolinalool, snake bed enema, snake Bed alkenol, 1.3.3-menthol triene, linalool, p-cymene. Conclusion: The paper first explores the antioxidant fingerprint of Shennongxiangju volatile oil, and provides the scientific basis and references for the basic research, efficacy evaluation and quality standardization of the medicinal material of Shennongxiangju.