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目的:观察中国苏皖地区汉族老年(≥60岁)人群KLOTHO基因第4外显子C-1818T多态性分布,探讨其与老年人急性冠脉综合征(ACS)的可能关系。方法:采用聚合酶链反应及基因芯片技术,检测272例老年ACS患者(ACS组)和139例老年非冠心病者(对照组)的KLOTHO基因C-1818T多态性。结果:(1)与对照组相比:ACS组CT基因型频率明显升高,差异有统计学意义(30.9vs20.9,P=0.031),CC、TT基因型及T等位基因频率差异均无统计学意义(分别为72.7vs65.4、6.5vs3.7和16.9vs19.1,P值均>0.05)。(2)将ACS组按性别进行进一步分析后发现,分别与对照组相比:①男性CT基因型频率明显升高,差异有统计学意义(28.5vs16.7,P=0.041),CC、TT基因型及T等位基因频率差异无统计学意义(分别为75.6vs68.5、7.7vs3.0和16.0vs17.3,P值均>0.05);②女性CC、CT、TT基因型及T等位基因频率差异无统计学意义(分别为68.9vs56.9、26.2vs37.5、4.9vs5.6和18.0vs24.3,P值均>0.05)。在调整相关危险因素并经多元Logistic回归分析后,与对照组相比:ACS组(优势比=1.993,95可信区间=1.180-3.367,P=0.010)和ACS组男性患者(优势比=2.156,95可信区间=1.010-4.602,P=0.047)CT基因型频率均显著升高,差异有统计学意义。结论:在中国苏皖地区汉族老年人群(特别是老年男性)中,ACS患者CT基因型频率升高,提示KLOTHO基因C-1818T多态性CT基因型与老年人特别是老年男性ACS的发病存在相关性。
Objective: To investigate the distribution of C-1818T polymorphism in exon 4 of KLOTHO gene in elderly Han people (≥60 years old) in Jiangsu and Anhui Provinces and to explore its possible relationship with acute coronary syndrome (ACS) in the elderly. Methods: Polymorphisms of KLOTHO gene C-1818T in 272 elderly ACS patients (ACS group) and 139 elderly non-CHD patients (control group) were detected by polymerase chain reaction and gene chip technology. Results: (1) Compared with the control group, the frequency of CT genotype in ACS group was significantly higher than that in control group (30.9 vs. 20.9, P = 0.031). The frequencies of CC, TT genotype and T allele There was no statistical significance (72.7 vs 65.4, 6.5 vs 3.7 and 16.9 vs 19.1, P> 0.05). (2) Further analysis of ACS group by sex found that compared with the control group respectively: ①The frequency of CT genotype in male was significantly higher than that in control group (28.5 vs16.7, P = 0.041), CC, TT The frequencies of genotype and T allele had no statistical significance (75.6 vs68.5, 7.7 vs 3.0, and 16.0 vs 17.3, respectively, P values> 0.05) .②The genotypes of CC, CT, TT and T There was no significant difference in the frequency of the gene (68.9 vs 56.9, 26.2 vs 37.5, 4.9 vs 5.6 and 18.0 vs 24.3 respectively, P> 0.05). After adjustment for risk factors and multivariate Logistic regression analysis, the ACS group (odds ratio = 1.993, 95% confidence interval = 1.180-3.367, P = 0.010) and ACS group (odds ratio = 2.156 , 95 credible interval = 1.010-4.602, P = 0.047). The frequencies of CT genotypes were significantly increased, the difference was statistically significant. Conclusions: The frequency of CT genotypes in ACS patients is higher in the Han Chinese elderly population (especially in older men) in Jiangsu and Anhui Provinces, which suggests that the CT genotype of KLOTHO gene C-1818T polymorphism is associated with the incidence of ACS in the elderly, especially in older men Correlation.