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目的剖析浑源黄芪根际微生物菌群结构特征,为研究道地黄芪品质形成提供科学依据。方法采用核糖体内转录间隔序列法(Automated ribosomal intergenic spacer analysis,ARISA)建立微生物菌群指纹图谱,分别用软件Gene Marker 2.2.0和PAST对获得的原始图谱进行加工处理与分析。结果与非根际土相比,种植2~3年的浑源黄芪根际微生物菌群结构发生了明显改变,主要表现为相似度降低和多样性增加;种植5年的微生物菌群结构与非根际菌群间相似度无显著差异,多样性趋于一致。与其他产地相比,浑源黄芪核心群体分类操作单元(OTUs)数较低,但相对丰富度较高。结论浑源黄芪根际微生物菌群结构与种植年限有关,且明显不同于其他产地。
Objective To analyze the structural characteristics of the rhizosphere microbial flora of Astragalus membranaceus and provide a scientific basis for the study of the quality of Radix Astragali. Methods Fingerprints of microflora were established by using ribosomal intergenic spacer analysis (ARISA). The original map was processed and analyzed by Gene Marker 2.2.0 and PAST respectively. Results Compared with the non-rhizosphere soil, the structure of rhizosphere microbes in Astragalus membranaceus changed significantly after planting for 2 to 3 years, mainly showing the decrease of similarity and the increase of diversity. The five-year structure of microbial flora There was no significant difference in rhizobacteria between groups, and the diversity tended to be the same. Compared with other producing areas, the populations of Astragalus membranaceus from Hunyuan were lower in number of OTUs but higher in relative abundance. Conclusion The structure of rhizosphere microorganisms in Astragalus membranaceus is related to the planting years and obviously different from other origins.