论文部分内容阅读
目的 研究中枢神经系统感染时血管内皮细胞E选择素的表达 ,以及地塞米松对其抑制作用。方法 将 5 4只小鼠随机分成侧脑室注射内毒素 (2 0 μg/2 0 μl)组、腹腔注射地塞米松 [1mg/(kg·12h) ]+侧脑室注射内毒素 (2 0 μg/2 0 μl)组、生理盐水组以及空白对照组。免疫组织化学法观察脑血管内皮细胞E选择素的表达 ,HE染色观察脑组织炎症反应。结果 空白对照组有少量E选择素表达 [(13 7± 2 7) %]。生理盐水组 [(13 5± 5 6 ) %]与空白对照组E选择素表达比较差异无显著性。内毒素组注射后E选择素表达在第 1天 [(31 8± 10 5 ) %]、第 2天 [(2 6 8± 9 6 ) %]明显增多 ,与生理盐水组比较差异有显著性 (P <0 0 1或 <0 0 5 ) ,注射后第 3天 [(15 7± 9 9) %]恢复正常 (P >0 0 5 ) ;注射后 3d内E选择素表达呈进行性下降 (F =4 0 8,P <0 0 5 )。地塞米松 +内毒素组于注射后第 1天和第 2天E选择素表达与内毒素组比较明显受到抑制 ,分别为 (13 0± 12 4 ) %、(8 8±6 0 ) %(P略 >0 0 5 ,P <0 0 5 ) ,与生理盐水组比较差异无显著性 ;同时脑组织炎症反应受到明显抑制。结论 中枢神经系统感染时脑血管内皮细胞E选择素的表达增加 ;地塞米松可通过抑制脑血管内皮细胞表面E选择素的表达来抑制炎症反应?
Objective To investigate the expression of E-selectin in vascular endothelial cells during central nervous system infection and the inhibitory effect of dexamethasone. Methods 5 4 mice were randomly divided into 4 groups: intracerebroventricular (icv) injection of endotoxin (20 μg / 20 μl), intraperitoneal injection of dexamethasone [1 mg / (kg · 12 h)] + 2 0 μl) group, saline group and blank control group. The expression of E-selectin in cerebrovascular endothelial cells was observed by immunohistochemistry and the inflammatory reaction in brain tissue was observed by HE staining. Results There was a small amount of E-selectin expression in the blank control group [(13 7 ± 2 7)%]. There was no significant difference in the expression of E-selectin between the saline group [(13 5 ± 5 6)%] and the blank control group. The expression of E-selectin in the endotoxin group was significantly higher than that in the saline group at day 1 [(31 8 ± 10 5)%] [day 2 (2 6 8 ± 96)%] (P <0.01 or <0.05), and returned to normal on the third day after injection (P <0.05). The expression of E-selectin decreased progressively after 3d (F = 4 0 8, P <0 0 5). The levels of E-selectin in dexamethasone group and endotoxin group were significantly lower than those in the endotoxin group at day 1 and day 2, respectively (13 0 ± 12 4%, (8 8 ± 60)% P slightly> 0.05, P <0 05), no significant difference compared with the saline group; at the same time, brain inflammation was significantly inhibited. Conclusion Dexamethasone can inhibit E-selectin expression in cerebrovascular endothelial cells by inhibiting the expression of E-selectin on the surface of cerebrovascular endothelial cells.