目的探讨大蒜素(AL)保护氧糖剥夺再灌注(OGD/RP)致PC12细胞损伤的抗氧化应激机制。方法采用无糖的Earle’s平衡盐溶液加缺氧法复制氧糖剥夺再灌注致PC12细胞损伤模型,同时给予不同浓度的大蒜素进行干预。采用MTT法检测细胞存活率;化学比色法测定细胞乳酸脱氢酶(LDH)漏出率;Hoechst 33342染色法检测大蒜素对细胞凋亡的影响,并计算凋亡率;生化法检测细胞超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)的活性或含量;RT-PCR和Western blotting分子生物学方法检测氧化应激相关因子SOD、肿瘤坏死因子(TNF-α)、过氧化氢酶(CAT)mRNA和蛋白的表达。结果与正常对照组比较,OGD/RP模型组PC12细胞存活率下降,LDH漏出率、细胞凋亡率明显升高;细胞SOD、GSH-Px活性明显降低,MDA含量明显升高;细胞SOD、CAT mRNA及蛋白表达明显下降,TNF-αmRNA及蛋白表达明显增加。与模型组比较,大蒜素(60、30mg/L)可显著升高OGD/RP PC12细胞的存活率,降低凋亡率和LDH漏出率;大蒜素(60、30mg/L)可显著升高OGD/RP PC12细胞SOD、GSH-Px活性,减少MDA含量;大蒜素(60mg/L)可明显升高OGD/RP PC12细胞SOD、CAT mRNA及蛋白表达,降低TNF-αmRNA及蛋白表达。结论大蒜素对OGD/RP致PC12细胞损伤具有保护作用,其机制与抗氧化应激有关。 Objective To investigate the anti-oxidative stress mechanism of allicin (AL) protecting PC12 cells from oxidative stress-deprivation and reperfusion injury (OGD / RP). Methods The non-sugar Earle’s balanced salt solution plus hypoxia was used to replicate the model of PC12 cell injury induced by oxygen deprivation and reperfusion. At the same time, different concentrations of allicin were administrated. The cell viability was measured by MTT assay. The leakage rate of lactate dehydrogenase (LDH) was measured by chemiluminescence assay. The effect of allicin on apoptosis was detected by Hoechst 33342 staining and the apoptosis rate was calculated. (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were detected by RT-PCR and Western blotting methods. Tumor necrosis factor (TNF-α), catalase (CAT) mRNA and protein expression. Results Compared with the normal control group, the survival rate of PC12 cells in OGD / RP group was decreased, the rate of LDH leakage and apoptosis were significantly increased, the activity of SOD and GSH-Px was significantly decreased and the content of MDA was significantly increased. The activities of SOD, CAT mRNA and protein expression decreased significantly, TNF-αmRNA and protein expression increased significantly. Compared with the model group, allicin (60 and 30 mg / L) significantly increased the survival rate of OGD / RP PC12 cells and decreased the rate of apoptosis and LDH leakage rate; allicin (60 and 30 mg / L) significantly increased OGD / RP PC12 cells, and decreased the content of MDA. Allicin (60 mg / L) significantly increased the expression of SOD, CAT mRNA and protein and decreased the expression of TNF-α mRNA and protein in OGD / RP PC12 cells. Conclusion Allicin has a protective effect on PC12 cells induced by OGD / RP. The mechanism is related to anti-oxidative stress.