论文部分内容阅读
脊椎动物中枢神经系统中快激动性神经传递主要是由L-谷氨酸(Glu)介导的。Glu的离子型受体,在药理、生理和激动剂结合这些性质基础上可分类为NMDA(N-甲基-D-天冬氨酸),AMPA(α-氨基-3-羟基-5-甲基-噁唑-4-丙酸)和KA(海人酸)亚型。在对编码Glu非NMDA受体亚基的cDNA克隆序列作比较后可发现:至少有两类亚基:G1uR1-GluR 4和GluR 5。作者现已克隆和表达了一个新的功能性Glu受体亚基——GluR6。它与G1uR1-GluR4有十分不同的药理特性。作者用Glu受体GluR5 cDNA克隆的编码区作探针,从大鼠小脑cDNA库中分离出一个有4 559个
Fast-acting neurotransmission in the vertebrate central nervous system is mainly mediated by L-glutamic acid (Glu). Glu ionotropic receptors, which can be classified into NMDA (N-methyl-D-aspartic acid), AMPA (α-amino-3-hydroxy- Yl-oxazole-4-propionic acid) and KA (kainate) subtype. Comparisons of cDNA cloning sequences encoding Glu non-NMDA receptor subunits revealed that there are at least two subtypes GluR1-GluR4 and GluR5. The authors have now cloned and expressed a new functional Glu receptor subunit, GluR6. It has very different pharmacological properties from G1uR1-GluR4. The authors used Glu receptor GluR5 cDNA clone coding region as a probe from the rat cerebellum cDNA library isolated a 4 559